Pillay D, Charman H, Lok J, Griffiths P D
Division of Communicable Diseases, Royal Free Hospital and School of Medicine, London, UK.
J Virol Methods. 1992 Nov;40(2):219-24. doi: 10.1016/0166-0934(92)90070-t.
A monoclonal antibody cocktail, directed against immediate early antigens of cytomegalovirus, was assessed in a rapid culture system for detection of CMV (DEAFF) in clinical samples. The antibody cocktail was shown to be equivalent in sensitivity and specificity to our currently used monoclonal antibody. A comparison of DEAFF (using either monoclonal preparation) and conventional cell culture shows that DEAFF detects a smaller proportion of CMV positive samples from those receiving antiviral chemotherapy. The reasons for the discrepancies observed between these techniques are discussed.
一种针对巨细胞病毒即刻早期抗原的单克隆抗体混合物，在一种快速培养系统中进行评估，用于检测临床样本中的巨细胞病毒（DEAFF）。结果表明，该抗体混合物在敏感性和特异性方面与我们目前使用的单克隆抗体相当。对DEAFF（使用任何一种单克隆制剂）和传统细胞培养进行比较发现，DEAFF从接受抗病毒化疗的患者中检测到的巨细胞病毒阳性样本比例较小。本文讨论了这些技术之间观察到差异的原因。
