慢性巨细胞病毒感染

Chronic cytomegalovirus infection.

作者信息

Griffiths P D

机构信息

Department of Virology, Royal Free Hospital School of Medicine, London, U.K.

出版信息

J Virol Methods. 1988 Sep;21(1-4):79-86. doi: 10.1016/0166-0934(88)90054-7.

DOI:10.1016/0166-0934(88)90054-7

PMID:2846622

Abstract

Cytomegalovirus infects about 60% of persons in developed countries and may cause significant disease in immunosuppressed patients. Serology has no role in the diagnosis of CMV disease in such patients yet detection of the virus in diseased parts of the body can be used to monitor the course of disease and antiviral measures. Detection of viral proteins (alpha or beta proteins) by monoclonal antibodies in cell cultures infected by urine, saliva or blood samples from patients allows a positive diagnosis within 24 h (DEAFF test). Monoclonal antibodies against CMV can also be used to stain cells obtained directly from the infected patients.

摘要

在发达国家，约60%的人感染巨细胞病毒，且该病毒可能在免疫抑制患者中引发严重疾病。血清学检测在这类患者的巨细胞病毒疾病诊断中并无作用，但在身体患病部位检测到该病毒可用于监测疾病进程及抗病毒措施。通过单克隆抗体在受患者尿液、唾液或血液样本感染的细胞培养物中检测病毒蛋白（α或β蛋白），可在24小时内做出阳性诊断（DEAFF检测）。针对巨细胞病毒的单克隆抗体也可用于对直接从感染患者获取的细胞进行染色。