通过原位杂交对犬猫细小病毒肠炎进行特异性诊断。

Specific diagnosis of parvovirus enteritis in dogs and cats by in situ hybridization.

作者信息

Waldvogel A S, Hassam S, Stoerckle N, Weilenmann R, Tratschin J D, Siegl G, Pospischil A

机构信息

Institut für Veterinärpathologie, Universität Zürich, Switzerland.

出版信息

J Comp Pathol. 1992 Aug;107(2):141-6. doi: 10.1016/0021-9975(92)90031-o.

DOI:10.1016/0021-9975(92)90031-o

PMID:1333495

Abstract

In a retrospective study, the fixed intestines of 10 dogs and 10 cats with intestinal lesions characteristic of parvovirus infection were assayed for the presence of parvovirus by in situ hybridization and immunohistochemistry. Parvoviral nucleic acid was localized by in situ hybridization in intestinal tissue in all 10 dogs and in nine of the 10 cats, whereas antigen was detected only in seven of 10 canine and eight of 10 feline intestines by immunohistochemistry. We conclude that an aetiological diagnosis can be established with a high degree of certainty by routine histology. Demonstration of the infectious agent by in situ hybridization, however, proves to be a valuable specific tool which allows an exact cellular localization of parvovirus in formalin-fixed, paraffin wax-embedded tissue sections.

摘要

在一项回顾性研究中，对10只患有细小病毒感染特征性肠道病变的犬和10只猫的固定肠道组织，通过原位杂交和免疫组织化学检测细小病毒的存在情况。通过原位杂交，在所有10只犬和10只猫中的9只的肠道组织中定位到了细小病毒核酸，而通过免疫组织化学仅在10只犬中的7只和10只猫中的8只的肠道中检测到了抗原。我们得出结论，通过常规组织学检查可以高度确定地做出病因诊断。然而，原位杂交显示感染因子被证明是一种有价值的特异性工具，它能够在福尔马林固定、石蜡包埋的组织切片中对细小病毒进行精确的细胞定位。