Nho W G, Sur J H, Doster A R, Kim S B
Laboratory of Pathology, Pusan Veterinary Service Laboratory, Buk-Ku, South Korea.
J Vet Diagn Invest. 1997 Jul;9(3):255-60. doi: 10.1177/104063879700900306.
An improved method for the diagnosis of canine parvovirus using in situ hybridization in standard formalin-fixed, paraffin-embedded tissue sections was developed. A digoxigenin-labeled probe complementary to DNA sequences that code for the entire sequence of the capsid protein VP-1 and the middle part of the sequence of the capsid protein VP-2 was designed. Specific histologic localization of canine parvovirus-infected cells was demonstrated in small intestine, tonsil, lymph node, thymus, spleen, heart, liver, and kidney from dogs diagnosed at necropsy with canine parvovirus infection. The in situ hybridization accurately pinpointed the specific sites of viral infection. The detection of canine parvovirus in liver, kidney, and heart tissues together in the same pups could represent an enhanced virulence of this strain of canine parvovirus and suggests a broadened tissue tropism not seen before in Korean strains of canine parvovirus.
开发了一种在标准福尔马林固定、石蜡包埋组织切片中使用原位杂交诊断犬细小病毒的改进方法。设计了一种地高辛标记的探针，该探针与编码衣壳蛋白VP-1的整个序列和衣壳蛋白VP-2序列中间部分的DNA序列互补。在尸检诊断为犬细小病毒感染的犬的小肠、扁桃体、淋巴结、胸腺、脾脏、心脏、肝脏和肾脏中，证实了犬细小病毒感染细胞的特异性组织学定位。原位杂交准确地确定了病毒感染的特定部位。在同一幼犬的肝脏、肾脏和心脏组织中同时检测到犬细小病毒，可能代表该株犬细小病毒的毒力增强，并提示韩国犬细小病毒株以前未见的组织嗜性扩大。
