Walter J, Dohse K, Rudolph R
Zentralbl Veterinarmed B. 1989 Jul;36(5):321-32.
A modified ABC-method (avidin-biotin-peroxidase complex) is established to demonstrate viral antigens in paraffin sections of tissues of coronavirus (FIP) infected cats and parvovirus type 2 infected dogs using monoclonal antibodies. The pros and cons and potential sources of error are described and discussed.
建立了一种改良的ABC法(抗生物素蛋白-生物素-过氧化物酶复合物法),使用单克隆抗体在冠状病毒(猫传染性腹膜炎)感染猫和2型细小病毒感染犬的组织石蜡切片中显示病毒抗原。描述并讨论了该方法的优缺点及潜在的误差来源。