Fritsche K L, Cassity N A
Department of Animal Sciences, University of Missouri-Columbia 65211.
Poult Sci. 1992 Oct;71(10):1646-57. doi: 10.3382/ps.0711646.
The overall goal of the present study was to determine whether the incorporation of n-3 fatty acids into poultry rations would alter the immune response of broiler chickens. Female broiler chicks were fed a corn and soybean meal-based diet to which one of four dietary fats were added: lard (LA), corn oil (CO), flaxseed oil (SO), or menhaden fish oil (FO). The latter two fat sources are rich in n-3 polyunsaturated fatty acids (PUFA). Enriching the diet with n-3 PUFA did not alter the primary or secondary antibody response of broiler chickens to sheep red blood cells. Dietary fat source had no effect on antibody-dependent cell cytotoxicity (ADCC) by peripheral blood leukocytes, but ADCC by splenocytes was 50% lower in chickens fed SO and FO compared with LA and CO (P less than .005). As expected, the fatty acid profile of the isolated immune cells reflected the fatty acid composition of the dietary fats fed. Basal release and calcium ionophore (A23187)-stimulated (10 microM) release of thromboxane B were significantly lower (P less than .05) in the SO and FO groups compared with the LA and CO groups. Total leukotriene B release was not significantly altered by dietary fat source. In conclusion, feeding broiler chickens diets rich in n-3 PUFA reduced ADCC of splenocytes and altered eicosanoid production by isolated immune cells.
本研究的总体目标是确定在禽类日粮中添加n-3脂肪酸是否会改变肉鸡的免疫反应。给雌性肉雏鸡饲喂以玉米和豆粕为基础的日粮，并添加以下四种日粮脂肪之一：猪油（LA）、玉米油（CO）、亚麻籽油（SO）或鲱鱼油（FO）。后两种脂肪来源富含n-3多不饱和脂肪酸（PUFA）。用n-3 PUFA强化日粮并未改变肉鸡对绵羊红细胞的初次或二次抗体反应。日粮脂肪来源对外周血白细胞的抗体依赖性细胞毒性（ADCC）没有影响，但与饲喂LA和CO的鸡相比，饲喂SO和FO的鸡的脾细胞ADCC降低了50%（P小于0.005）。正如预期的那样，分离出的免疫细胞的脂肪酸谱反映了所喂日粮脂肪的脂肪酸组成。与LA和CO组相比，SO和FO组的血栓素B基础释放量和钙离子载体（A23187）刺激（10 microM）释放量显著降低（P小于0.05）。日粮脂肪来源对总白三烯B释放量没有显著影响。总之，给肉鸡饲喂富含n-3 PUFA的日粮会降低脾细胞的ADCC，并改变分离出的免疫细胞的类花生酸生成。
