Fritsche K L, Alexander D W, Cassity N A, Huang S C
Department of Animal Sciences, University of Missouri, Columbia 65211.
Lipids. 1993 Aug;28(8):677-82. doi: 10.1007/BF02535986.
This study was designed to examine the incorporation of omega-3 (n-3) fatty acids into the immune tissues of pigs nursing fish oil-fed sows and to determine the effect of maternal dietary n-3 consumption on in vitro immune cell eicosanoid production. On day 107 of gestation, 12 sows were randomly allotted to a diet containing either 7% menhaden fish oil (MFO) or lard (LRD). The fatty acid profile of serum, liver, thymus, splenocytes and alveolar macrophages (AM) of 18-21-day-old pigs was significantly affected by the fat source provided to the sow. Arachidonic acid (20:4n-6) content was typically reduced by more than 50% in MFO as compared with LRD pigs. In MFO pigs, eicosapentaenoic acid (20:5n-3) was the major n-3 polyunsaturated fatty acid, and its levels matched or exceeded those of arachidonic acid. Basal release of prostaglandin E, thromboxane B and leukotriene B by AM was 60-70% lower in MFO vs. LRD pigs. However, when these immune cells were stimulated with calcium ionophore A23187, release of leukotriene B was similar in MFO and LRD pigs. In conclusion, substituting MFO for LRD in a sow's late-gestation and lactation diet greatly elevated the content of n-3 fatty acids in the nursing pig immune cells and generally reduced in vitro eicosanoid release by pig immune cells.
本研究旨在检测ω-3（n-3）脂肪酸在食用鱼油的母猪所哺育仔猪免疫组织中的掺入情况，并确定母体日粮中n-3脂肪酸摄入量对体外免疫细胞类花生酸生成的影响。在妊娠第107天，将12头母猪随机分为两组，分别饲喂含7%鲱鱼油（MFO）或猪油（LRD）的日粮。母猪所提供的脂肪来源对18 - 21日龄仔猪的血清、肝脏、胸腺、脾细胞和肺泡巨噬细胞（AM）的脂肪酸谱有显著影响。与LRD组仔猪相比，MFO组仔猪的花生四烯酸（20:4n-6）含量通常降低了50%以上。在MFO组仔猪中，二十碳五烯酸（20:5n-3）是主要的n-3多不饱和脂肪酸，其水平与花生四烯酸相当或超过花生四烯酸。与LRD组仔猪相比，MFO组仔猪AM中前列腺素E、血栓素B和白三烯B的基础释放量降低了60 - 70%。然而，当用钙离子载体A23187刺激这些免疫细胞时，MFO组和LRD组仔猪白三烯B的释放量相似。总之，在母猪妊娠后期和泌乳期日粮中用MFO替代LRD可显著提高哺乳仔猪免疫细胞中n-3脂肪酸的含量，并普遍降低猪免疫细胞体外类花生酸的释放。
