Lyaku J R, Sinclair J A, Nettleton P F, Marsden H S
Moredun Research Institute, Edinburgh, UK.
Vet Microbiol. 1992 Oct;32(3-4):229-39.
Fourteen hybridoma cell lines secreting monoclonal antibodies (Mab) to cervine herpesvirus-1 (CerHV-1) produced following the fusion of NSO myeloma cells with splenocytes of BALB/c mice previously immunized with gradient purified CerHV-1 were selected using an indirect enzyme-linked immunosorbent assay (ELISA) employing CerHV-1 antigen and tested by the ELISA against four other ruminant alphaherpesviruses from cattle (bovine herpesvirus type 1.1 and 1.2) goat (caprine herpesvirus-2) and reindeer (rangiferine herpesvirus-1). Comparison of all five ruminant alphaherpesviruses with these Mabs confirmed their close antigenic relationships, with two Mabs reacting against all viruses. Ten Mabs which were able to differentiate between the viruses reacted with a 64 kDa polypeptide in a western blot. Four Mabs including two specific only for CerHV-1 with neutralizing activity against the virus used for immunization were directed against a 74 kDa viral protein.
用梯度纯化的鹿疱疹病毒-1（CerHV-1）免疫BALB/c小鼠，取其脾细胞与NSO骨髓瘤细胞融合后，通过间接酶联免疫吸附测定（ELISA），利用CerHV-1抗原筛选出14株分泌抗鹿疱疹病毒-1单克隆抗体（Mab）的杂交瘤细胞系，并用ELISA针对来自牛（牛疱疹病毒1.1型和1.2型）、山羊（山羊疱疹病毒-2）和驯鹿（驯鹿疱疹病毒-1）的其他四种反刍动物α疱疹病毒进行检测。用这些单克隆抗体对所有五种反刍动物α疱疹病毒进行比较，证实了它们密切的抗原关系，其中两种单克隆抗体能与所有病毒发生反应。在蛋白质印迹法中，10种能够区分这些病毒的单克隆抗体与一种64 kDa的多肽发生反应。包括两种仅对CerHV-1具有特异性且对用于免疫的病毒具有中和活性的单克隆抗体在内的四种单克隆抗体针对一种74 kDa的病毒蛋白。
