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Stimulation of thymidine kinase activity in baculovirus infected cells is not due to a virus-coded enzyme.

作者信息

Wang X, Xie W, Long Q, He D, Lin G, Pang Y, Pu Z

机构信息

Institute of Entomology, Zhongshan University, Guangzhou, People's Republic of China.

出版信息

Arch Virol. 1992;127(1-4):315-9. doi: 10.1007/BF01309593.

DOI:10.1007/BF01309593
PMID:1333755
Abstract

A polyhedrin-positive recombinant autographa californica nuclear polyhedrosis virus (AcNPV) carrying a herpes simplex virus thymidine kinase gene under the control of the SynXIV promoter, a fusion of synthetic and linker-modified polyhedrin promoters, has been constructed. When this recombinant baculovirus was used to infect a variant of Spodoptera frugiperda cells deficient in thymidine kinase (TK-), a high level of TK activity was detected. These results, in conjunction with the demonstration that AcNPV could replicate in TK- S. frugiperda cells and no TK activity was found throughout infection, imply that the wild type virus-stimulated TK activity observed in S. frugiperda (TK+) cells is not contributed by a virus-coded enzyme.

摘要

相似文献

1
Stimulation of thymidine kinase activity in baculovirus infected cells is not due to a virus-coded enzyme.
Arch Virol. 1992;127(1-4):315-9. doi: 10.1007/BF01309593.
2
Replication inhibition by nucleoside analogues of a recombinant Autographa californica multicapsid nuclear polyhedrosis virus harboring the herpes thymidine kinase gene driven by the IE-1(0) promoter: a new way to select recombinant baculoviruses.由携带由IE-1(0)启动子驱动的疱疹胸苷激酶基因的重组苜蓿银纹夜蛾多粒包埋核型多角体病毒的核苷类似物介导的复制抑制：一种选择重组杆状病毒的新方法。
Nucleic Acids Res. 1992 Dec 11;20(23):6239-46. doi: 10.1093/nar/20.23.6239.
3
Expression of cauliflower mosaic virus gene I in insect cells using a novel polyhedrin-based baculovirus expression vector.使用一种基于新型多角体蛋白的杆状病毒表达载体在昆虫细胞中表达花椰菜花叶病毒基因I。
J Gen Virol. 1990 Oct;71 ( Pt 10):2201-9. doi: 10.1099/0022-1317-71-10-2201.
4
Heterologous recombination between Autographa californica nuclear polyhedrosis virus DNA and foreign DNA in non-polyhedrin segments of the viral genome.苜蓿银纹夜蛾核型多角体病毒DNA与病毒基因组非多角体蛋白区段中的外源DNA之间的异源重组。
Virus Res. 1991 Sep;21(1):65-85. doi: 10.1016/0168-1702(91)90072-4.
5
Expression of the Autographa californica nuclear polyhedrosis virus p10 gene: effect of polyhedrin gene expression.苜蓿银纹夜蛾核型多角体病毒p10基因的表达：多角体蛋白基因表达的影响
Arch Virol. 1992;123(1-2):1-11. doi: 10.1007/BF01317134.
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A baculovirus dual expression vector derived from the Autographa californica nuclear polyhedrosis virus polyhedrin and p10 promoters: co-expression of two influenza virus genes in insect cells.一种源自苜蓿银纹夜蛾核型多角体病毒多角体蛋白和p10启动子的杆状病毒双表达载体：两种流感病毒基因在昆虫细胞中的共表达。
J Gen Virol. 1991 Dec;72 ( Pt 12):2967-74. doi: 10.1099/0022-1317-72-12-2967.
7
Expression of plant virus genes in animal cells: high-level synthesis of cowpea mosaic virus B-RNA-encoded proteins with baculovirus expression vectors.植物病毒基因在动物细胞中的表达：利用杆状病毒表达载体高效合成豇豆花叶病毒B-RNA编码蛋白
J Gen Virol. 1990 Nov;71 ( Pt 11):2509-17. doi: 10.1099/0022-1317-71-11-2509.
8
Transcriptional analyses of baculovirus polyhedrin and foreign gene expression relative to baculovirus p10 mRNA levels.杆状病毒多角体蛋白和外源基因表达相对于杆状病毒p10 mRNA水平的转录分析。
J Gen Virol. 1991 Oct;72 ( Pt 10):2551-6. doi: 10.1099/0022-1317-72-10-2551.
9
Development of baculovirus triple and quadruple expression vectors: co-expression of three or four bluetongue virus proteins and the synthesis of bluetongue virus-like particles in insect cells.杆状病毒三重和四重表达载体的构建：三种或四种蓝舌病毒蛋白的共表达以及在昆虫细胞中合成蓝舌病毒样颗粒
Nucleic Acids Res. 1993 Mar 11;21(5):1219-23. doi: 10.1093/nar/21.5.1219.
10
Proteins encoded in the 81.2- to 85.0-map-unit fragment of Autographa californica nuclear polyhedrosis virus DNA can be translated in vitro and in Spodoptera frugiperda cells.苜蓿银纹夜蛾核型多角体病毒DNA的81.2至85.0图谱单位片段中编码的蛋白质能够在体外和草地贪夜蛾细胞中进行翻译。
J Virol. 1991 Jan;65(1):89-97. doi: 10.1128/JVI.65.1.89-97.1991.

本文引用的文献

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Baculovirus induction of a DNA polymerase.杆状病毒诱导的一种DNA聚合酶。
J Virol. 1981 Oct;40(1):305-8. doi: 10.1128/JVI.40.1.305-308.1981.
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Production of human beta interferon in insect cells infected with a baculovirus expression vector.在感染杆状病毒表达载体的昆虫细胞中生产人β干扰素。
Mol Cell Biol. 1983 Dec;3(12):2156-65. doi: 10.1128/mcb.3.12.2156-2165.1983.
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Vaccinia virus: a selectable eukaryotic cloning and expression vector.痘苗病毒：一种可选择的真核克隆和表达载体。
Proc Natl Acad Sci U S A. 1982 Dec;79(23):7415-9. doi: 10.1073/pnas.79.23.7415.
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Mapping of the vaccinia virus thymidine kinase gene by marker rescue and by cell-free translation of selected mRNA.通过标记拯救和对选定信使核糖核酸的无细胞翻译来定位牛痘病毒胸苷激酶基因。
Proc Natl Acad Sci U S A. 1982 Feb;79(4):1210-4. doi: 10.1073/pnas.79.4.1210.
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The nucleotide sequence and transcript map of the herpes simplex virus thymidine kinase gene.单纯疱疹病毒胸苷激酶基因的核苷酸序列及转录图谱。
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Origin of the thymidine kinase induced by polyoma virus in productively infected cells.多瘤病毒在有效感染细胞中诱导产生的胸苷激酶的起源。
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The location, sequence, transcription, and regulation of a baculovirus DNA polymerase gene.杆状病毒DNA聚合酶基因的定位、序列、转录及调控
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Baculovirus vectors for multiple gene expression and for occluded virus production.用于多基因表达和产生包涵体病毒的杆状病毒载体。
Gene. 1991 Apr;100:131-7. doi: 10.1016/0378-1119(91)90358-i.
9
Lepidopteran cell variants resistant to 5-bromodeoxyuridine and their use for transfection of the HSV-TK gene.对5-溴脱氧尿苷具有抗性的鳞翅目细胞变体及其用于单纯疱疹病毒胸苷激酶（HSV-TK）基因转染的用途。
J Cell Sci. 1991 Sep;100 ( Pt 1):243-7. doi: 10.1242/jcs.100.1.243.
10
Role of viral ribonucleotide reductase in the increase of dTTP pool size in herpes simplex virus-infected Vero cells.病毒核糖核苷酸还原酶在单纯疱疹病毒感染的非洲绿猴肾细胞中增加脱氧胸苷三磷酸池大小的作用。
J Gen Virol. 1991 Jun;72 ( Pt 6):1441-4. doi: 10.1099/0022-1317-72-6-1441.