Belyaev A S, Roy P
Laboratory of Molecular Biophysics, University of Oxford, UK.
Nucleic Acids Res. 1993 Mar 11;21(5):1219-23. doi: 10.1093/nar/21.5.1219.
Baculovirus multiple gene transfer vectors pAcAB3 and pAcAB4 have been developed to facilitate the insertion of three or four foreign genes respectively into the Autographa californica nuclear polyhedrosis virus (AcNPV) genome by a single co-transfection experiment. The pAcAB3 vector contains a polyhedrin promoter and two p10 promoters on either side of the polyhedrin promoter but in opposite orientations. The pAcAB4 vector has an additional polyhedrin promoter in opposite orientation to the first copy that is in juxtaposition to the first p10 promoter. Each of these derived vectors (pAcAB3, pAcAB4) have been used for the simultaneous expression of three or four bluetongue virus (BTV) genes respectively. When Spodoptera frugiperda cells were infected with the recombinant virus (AcBT-3/2/7/5) expressing the four major structural genes of BTV, double-capsid, virus-like particles consisting of VP2, VP3, VP5 and VP7 of BTV were assembled.
杆状病毒多基因转移载体pAcAB3和pAcAB4已被开发出来，以便通过单次共转染实验分别将三个或四个外源基因插入苜蓿银纹夜蛾核型多角体病毒（AcNPV）基因组。pAcAB3载体在多角体蛋白启动子两侧包含一个多角体蛋白启动子和两个p10启动子，但方向相反。pAcAB4载体在与第一个p10启动子并列的第一个拷贝的相反方向上有一个额外的多角体蛋白启动子。这些衍生载体（pAcAB3、pAcAB4）中的每一个都已分别用于同时表达三个或四个蓝舌病毒（BTV）基因。当用表达BTV四个主要结构基因的重组病毒（AcBT - 3/2/7/5）感染草地贪夜蛾细胞时，由BTV的VP2、VP3、VP5和VP7组成的双壳病毒样颗粒被组装起来。
