Corrie J E, Pizza C, Makwana J, King R W
National Institute for Medical Research, London, United Kingdom.
Protein Expr Purif. 1992 Oct;3(5):417-20. doi: 10.1016/s1046-5928(05)80044-0.
Reaction of cyclic AMP with 1,1'-carbonyldiimidazole produces an intermediate which reacts with primary amines to provide a stable 2'-O-carbamyl derivative. This chemistry has been used to tether cyclic AMP to a Sepharose gel. The resulting affinity support has been used to effect a simple, nondenaturing purification of cyclic AMP receptor protein from crude cell extracts.
环磷酸腺苷（cAMP）与1,1'-羰基二咪唑反应生成一种中间体，该中间体与伯胺反应可生成稳定的2'-O-氨基甲酰基衍生物。这种化学方法已被用于将cAMP连接到琼脂糖凝胶上。所得的亲和载体已用于从粗细胞提取物中简单、非变性地纯化cAMP受体蛋白。
