Preparation and properties of an affinity support for purification of cyclic AMP receptor protein from Escherichia coli.
作者信息
Corrie J E, Pizza C, Makwana J, King R W
机构信息
National Institute for Medical Research, London, United Kingdom.
出版信息
Protein Expr Purif. 1992 Oct;3(5):417-20. doi: 10.1016/s1046-5928(05)80044-0.
PMID:1333861
Abstract
Reaction of cyclic AMP with 1,1'-carbonyldiimidazole produces an intermediate which reacts with primary amines to provide a stable 2'-O-carbamyl derivative. This chemistry has been used to tether cyclic AMP to a Sepharose gel. The resulting affinity support has been used to effect a simple, nondenaturing purification of cyclic AMP receptor protein from crude cell extracts.
摘要
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