Gerna G, Baldanti F, Zavattoni M, Sarasini A, Percivalle E, Revello M G
Virus Laboratory, University of Pavia, IRCCS Policlinico S. Matteo, Italy.
Antiviral Res. 1992 Oct 1;19(4):333-45. doi: 10.1016/0166-3542(92)90014-v.
A plaque-reduction assay for chemosensitivity testing of human cytomegalovirus (HCMV) strains was developed based on early detection of viral plaques 96 h p.i. by a monoclonal antibody to the major immediate-early protein p72. Sequential HCMV isolates from an AIDS patient undergoing multiple courses of ganciclovir treatment during an 18-month follow-up were tested by the new assay, showing emergence of a ganciclovir-resistant strain. However, cloning of viral isolates and Southern blot hybridization analysis showed the simultaneous presence of three different HCMV strains in blood. Of these, the resistant strain was likely to be selected during prolonged maintenance antiviral treatment, emerging during full drug regimen, while the two sensitive strains reappeared in association with the resistant one following drug discontinuation. This finding was demonstrated by high levels of ID90 and ID99 in sequential mixed viral populations. The new plaque assay leads to reduction in time needed for chemosensitivity testing and permits rapid tracing of drug-resistant strains in a mixed viral population.
基于在感染后96小时通过针对主要立即早期蛋白p72的单克隆抗体对病毒蚀斑进行早期检测，开发了一种用于人巨细胞病毒（HCMV）株化学敏感性测试的蚀斑减少试验。在18个月的随访期间，对一名接受多疗程更昔洛韦治疗的艾滋病患者的连续HCMV分离株进行了新试验检测，结果显示出现了一株对更昔洛韦耐药的毒株。然而，病毒分离株的克隆和Southern印迹杂交分析表明，血液中同时存在三种不同的HCMV毒株。其中，耐药毒株可能是在长期维持抗病毒治疗期间被选择出来的，在完整的药物治疗方案期间出现，而两种敏感毒株在停药后与耐药毒株一起重新出现。连续混合病毒群体中高水平的ID90和ID99证明了这一发现。新的蚀斑试验减少了化学敏感性测试所需的时间，并允许在混合病毒群体中快速追踪耐药毒株。
