大鼠细胞色素P-450 2B2基因启动子的分析
Analysis of the promoter of the cytochrome P-450 2B2 gene in the rat.
作者信息
Hoffmann M, Mager W H, Scholte B J, Civil A, Planta R J
机构信息
Department of Biochemistry and Molecular Biology, Vrije Universiteit, Amsterdam, The Netherlands.
出版信息
Gene Expr. 1992;2(4):353-63.
About 3 kb of the promoter region of the gene encoding cytochrome P-450 2B2 (CYP2B2) in the rat were sequenced and searched for potential cis-acting elements. Apart from putative binding sites for (liver-specific) protein factors, a region showing homology with the LINE 1 retrotransposon element was also found. Three proximal promoter fragments, encompassing nucleotides -579 to -372, -372 to -211, and -211 to +1, respectively, were shown to contain binding sites for multiple protein factors by bandshift analyses. The strongest protein-binding element, designated BRE (basic regulatory element), occurs between -103 to -66. Its structure is very similar to a negative control element in the murine cmyc promoter and displays a composite feature having a tandemly repeated sequence homology with the BTE (basic transcription element; Yanagida et al., 1990) separated by a CCAAA-box. The use of a deletion series of this template in in vitro transcription assays, provided evidence that the BRE serves as a major cis-acting element in the (regulated) transcription activation of the CYP2B2 gene.
对大鼠细胞色素P - 450 2B2（CYP2B2）编码基因的约3 kb启动子区域进行了测序，并寻找潜在的顺式作用元件。除了（肝脏特异性）蛋白质因子的假定结合位点外，还发现了一个与LINE 1逆转座子元件具有同源性的区域。通过带移分析表明，三个近端启动子片段，分别包含核苷酸-579至-372、-372至-211和-211至+1，含有多个蛋白质因子的结合位点。最强的蛋白质结合元件，称为BRE（基本调控元件），位于-103至-66之间。其结构与小鼠c - myc启动子中的一个负调控元件非常相似，并显示出一种复合特征，即与BTE（基本转录元件；柳田等人，1990）具有串联重复序列同源性，中间由一个CCAAA框隔开。在体外转录试验中使用该模板的缺失系列，提供了证据表明BRE作为CYP2B2基因（受调控的）转录激活中的主要顺式作用元件。
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