Yanagida A, Sogawa K, Yasumoto K I, Fujii-Kuriyama Y
Department of Chemistry, Faculty of Science, Tohoku University, Aobayama Sendai, Japan.
Mol Cell Biol. 1990 Apr;10(4):1470-5. doi: 10.1128/mcb.10.4.1470-1475.1990.
A novel cis-acting regulatory element (designated BTE for basic transcription element) was found in the region proximal to the TATA sequence of the P-450c gene by the use of deletion mutations. This DNA element is considered to be involved in the basic transcription of the gene and does not show distinct enhancer activity in itself. Together with the XRE sequence (A. Fujisawa-Sehara, K. Sogawa, M. Yamane, and Y. Fujii-Kuriyama, Nucleic Acids Res. 15:4179-4191, 1987), however, this sequence is required for a high inducible expression of the P-450c gene in response to xenobiotic inducers. The BTE sequence contained the GC box consensus sequence and half of the NF-1-binding consensus or CAT box sequence, but their synthetic oligonucleotides, used as competitors in the gel mobility shift assays, did not compete with the BTE sequence for the binding protein, suggesting that the BTE sequence functions as a different recognition sequence from that for Sp1 or NF-1. Analogous sequences to BTE are found in the region proximal to the TATA sequence of other genes, especially other P-450 genes with different modes of regulation, suggesting that the BTE sequence plays a common regulatory role in basic transcription of genes including a group of the P-450 superfamily. The ubiquitous distribution of nuclear factor(s) binding to this element supports this suggestion.
通过缺失突变，在P-450c基因TATA序列近端区域发现了一种新型顺式作用调节元件（命名为BTE，即基本转录元件）。该DNA元件被认为参与基因的基础转录，自身并不表现出明显的增强子活性。然而，与XRE序列（A. Fujisawa-Sehara、K. Sogawa、M. Yamane和Y. Fujii-Kuriyama，《核酸研究》15:4179 - 4191，1987）一起，该序列是P-450c基因对外源诱导剂产生高诱导表达所必需的。BTE序列包含GC盒共有序列以及NF-1结合共有序列或CAT盒序列的一半，但在凝胶迁移率变动分析中用作竞争剂的它们的合成寡核苷酸，并不与BTE序列竞争结合蛋白，这表明BTE序列作为一种与Sp1或NF-1不同的识别序列发挥作用。在其他基因的TATA序列近端区域发现了与BTE类似的序列，特别是其他具有不同调节模式的P-450基因，这表明BTE序列在包括一组P-450超家族基因的基础转录中发挥共同的调节作用。与该元件结合的核因子的普遍分布支持了这一观点。
