Keshavarzian A, Haydek J, Zabihi R, Doria M, D'Astice M, Sorenson J R
Department of Medicine, Loyola University Medical School, Maywood, Illinois.
Dig Dis Sci. 1992 Dec;37(12):1866-73. doi: 10.1007/BF01308081.
Reactive oxygen species (ROS) such as superoxide anion, hydrogen peroxide, hydroxyl radical, and hypochlorous acid have been implicated in the pathogenesis of inflammation and tissue injury in colitis. To determine whether or not anti-ROS agents can decrease the severity of colitis, we evaluated the effects of three known anti-ROS agents: catalase, WR-2721, and Cu(II)2(3,5-DIPS)4 on acetic acid-induced colonic inflammation in rats. Histologically, all three compounds significantly decreased the severity of colonic inflammation. The anti-ROS activity of these compounds was also tested using the luminol-enhanced chemiluminescence assay. Catalase, WR-2721, or Cu(II)2(3,5-DIPS)4 significantly inhibited luminol-enhanced chemiluminescence produced by inflamed colonic mucosa. These findings suggest that ROS, and in particular superoxide, hydrogen peroxide, and/or one of its secondarily derived species, may play an important role in acetic acid-induced colitis. Further studies are needed to determine the potential effectiveness of these compounds in human colitis.
活性氧（ROS），如超氧阴离子、过氧化氢、羟基自由基和次氯酸，已被认为与结肠炎的炎症和组织损伤发病机制有关。为了确定抗ROS药物是否能减轻结肠炎的严重程度，我们评估了三种已知抗ROS药物：过氧化氢酶、WR-2721和Cu(II)2(3,5-DIPS)4对大鼠乙酸诱导的结肠炎症的影响。组织学上，这三种化合物均显著降低了结肠炎症的严重程度。还使用鲁米诺增强化学发光法测试了这些化合物的抗ROS活性。过氧化氢酶、WR-2721或Cu(II)2(3,5-DIPS)4显著抑制了炎症结肠黏膜产生的鲁米诺增强化学发光。这些发现表明，ROS，特别是超氧阴离子、过氧化氢和/或其二次衍生的物种之一，可能在乙酸诱导的结肠炎中起重要作用。需要进一步研究以确定这些化合物在人类结肠炎中的潜在有效性。
