乳过氧化物酶产生单线态氧。
Singlet oxygen production by lactoperoxidase.
作者信息
Kanofsky J R
出版信息
J Biol Chem. 1983 May 25;258(10):5991-3.
PMID:6853474
Abstract
Infrared chemiluminescence with maximum intensity near 1270 nm was detected in the lactoperoxidase, H2O2, Br- system. No emission was observed when heat-inactivated enzyme was used or when Br- or H2O2 was deleted from the system. The chemiluminescence was increased by a factor of 42 +/- 5 (mean +/- S.E.) in 98% 2H2O. These results are strong evidence for the production of 1O2 in this system.
摘要
在乳过氧化物酶、H2O2、Br-体系中检测到最大强度接近1270 nm的红外化学发光。当使用热灭活的酶或从体系中去除Br-或H2O2时，未观察到发射。在98%的2H2O中，化学发光增加了42±5倍（平均值±标准误差）。这些结果有力地证明了该体系中产生了单线态氧。
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