Blondeau J M, Aoki F Y
Department of Medical Microbiology, University of Manitoba, Winnipeg, Canada.
J Virol Methods. 1992 Dec 1;40(3):323-9. doi: 10.1016/0166-0934(92)90090-z.
We have characterized previously a model of herpes simplex virus (HSV) infection of rat dorsal root ganglia (DRG) following cutaneous infection. During acute infection HSV can be isolated from co-cultivated rat ganglia in (mean +/- S.E.M.) 4.8 +/- 0.33 days (d) and from latently infected ganglia in 7.8 +/- 0.53 (d) (P < or = 0.0001). We treated co-cultivated rat ganglia from acute and latent infected rats with the demethylating compound hexamethylene-bis-acetamide (HEX) to see what effect, if any, it would have on HSV infection. HEX-treated ganglia from rats with acute infection did not differ significantly from controls in the proportion of rats, skin or ganglia positive for HSV. The mean time to detect virus was not different between treated (3.6 +/- 0.38 d) and control (3.1 +/- 0.46 d) (P > 0.05) groups. In latent infected rats there was no difference between treated and controlled groups in the proportions of rats, skin and ganglia positive for HSV. There was a significant difference in the mean time to CPE between the HEX and control groups respectively (4.5 +/- 0.72 d vs 8.92 +/- 1.42 d, P < 0.01). We conclude that HEX converted latent HSV infection to a productive one.
我们之前已对皮肤感染后大鼠背根神经节（DRG）的单纯疱疹病毒（HSV）感染模型进行了特征描述。在急性感染期间，HSV可在（平均±标准误）4.8±0.33天（d）从共培养的大鼠神经节中分离出来，而在潜伏感染的神经节中则在7.8±0.53（d）分离出来（P≤0.0001）。我们用去甲基化化合物六亚甲基双乙酰胺（HEX）处理来自急性和潜伏感染大鼠的共培养大鼠神经节，以观察其对HSV感染是否有影响。来自急性感染大鼠的经HEX处理的神经节在HSV阳性的大鼠、皮肤或神经节比例方面与对照组无显著差异。处理组（3.6±0.38 d）和对照组（3.1±0.46 d）检测到病毒的平均时间无差异（P＞0.05）。在潜伏感染的大鼠中，经处理组和对照组在HSV阳性的大鼠、皮肤和神经节比例方面无差异。HEX组和对照组出现细胞病变效应（CPE）的平均时间分别有显著差异（4.5±0.72 d对8.92±1.42 d，P＜0.01）。我们得出结论，HEX将潜伏性HSV感染转变为增殖性感染。
