Thomssen R, Bonk S, Propfe C, Heermann K H, Köchel H G, Uy A
Department of Medical Microbiology, University of Göttingen, Federal Republic of Germany.
Med Microbiol Immunol. 1992;181(5):293-300. doi: 10.1007/BF00198849.
Hepatitis C virus (HCV)-RNA in sera of patients with viral hepatitis C is supposed to be included, at least partially, into HCV particles. We found that the density of HCV-RNA-carrying material was variable, as determined by sucrose gradient density centrifugation (1.03-1.20 g/cm3). In some of the sera examined HCV-RNA was restricted to low densities between 1.03 and 1.08 g/cm3. In other sera additional densities of HCV-RNA were found distributed over the whole gradient with peaks at 1.12 and 1.17 and at 1.19-1.20 g/cm3. HCV-RNA banding at low densities could be completely co-precipitated with anti-beta lipoprotein, whereas HCV-RNA fractions of higher densities were only partially precipitated or not at all. In 8 of 20 sera directly examined, HCV-RNA could be completely and in 9 sera only partially co-precipitated by anti-beta lipoprotein. In 3 sera no significant precipitation could be observed.
丙型肝炎病毒（HCV）-RNA被认为至少部分地存在于丙型病毒性肝炎患者血清中的HCV颗粒中。我们发现，通过蔗糖梯度密度离心法测定（1.03-1.20 g/cm³），携带HCV-RNA物质的密度是可变的。在一些检测的血清中，HCV-RNA局限于1.03至1.08 g/cm³的低密度范围内。在其他血清中，发现HCV-RNA的其他密度分布在整个梯度中，峰值分别在1.12、1.17以及1.19-1.20 g/cm³处。低密度的HCV-RNA条带可与抗β脂蛋白完全共沉淀，而高密度的HCV-RNA部分仅部分沉淀或根本不沉淀。在直接检测的20份血清中，有8份血清中的HCV-RNA可被抗β脂蛋白完全共沉淀，9份血清中仅部分共沉淀。在3份血清中未观察到明显沉淀。
