Boyle A E, Smith B R, Amit Z
Center for Studies in Behavioral Neurobiology, Concordia University, Montreal, Quebec, Canada.
Pharmacol Biochem Behav. 1992 Dec;43(4):1121-7. doi: 10.1016/0091-3057(92)90491-w.
The effects of GABAA agonist THIP on the acquisition of voluntary ethanol intake and the pattern of food and water consumption were examined through the use of a computer-controlled data acquisition system. Twenty male Long-Evans rats were randomly assigned to two groups, one of which received THIP (16 mg/kg, IP) and the other an equal volume of saline. Subjects were presented with a free choice of ethanol and water immediately following drug injections, which occurred every other day. The initial concentration of ethanol presented was 2% and was increased by increments of 2% following the second presentation of each concentration, up to a maximum concentration of 10%. Subjects treated with THIP consumed significantly greater amounts of ethanol than did saline controls. A microstructural analysis of bout patterns suggested that the increased consumption of ethanol was a function of an increase in the size, duration, and frequency of ethanol drinking bouts. Food intake was also attenuated by THIP treatment. The results indicated that the decrease in total food intake was a function of a decrease in the frequency of the food bouts. However, in contrast to that observed for ethanol intake, the size and duration of the food bouts were unchanged. The qualitatively different patterns in the microstructure of consummatory behavior for ethanol and food following THIP treatment would suggest that differential mechanisms may mediate the food and ethanol effects observed in the present study. In addition, the differential effects of THIP on ethanol consumption relative to water would suggest that GABAA manipulations may play a role in influencing the acquisition of voluntary ethanol drinking.
通过使用计算机控制的数据采集系统，研究了GABAA激动剂THIP对自愿乙醇摄入量的获取以及食物和水消耗模式的影响。将20只雄性Long-Evans大鼠随机分为两组，一组接受THIP（16毫克/千克，腹腔注射），另一组接受等体积的生理盐水。在每隔一天进行的药物注射后，让实验对象自由选择乙醇和水。最初提供的乙醇浓度为2%，在每次浓度的第二次呈现后，每次以2%的增量增加，直至最大浓度10%。接受THIP治疗的实验对象比生理盐水对照组消耗的乙醇量显著更多。对饮酒发作模式的微观结构分析表明，乙醇消耗量的增加是乙醇饮酒发作的大小、持续时间和频率增加的结果。THIP治疗也使食物摄入量减少。结果表明，总食物摄入量的减少是食物发作频率降低的结果。然而，与乙醇摄入量的情况不同，食物发作的大小和持续时间没有变化。THIP治疗后，乙醇和食物的消费行为微观结构中质的不同模式表明，不同的机制可能介导了本研究中观察到的食物和乙醇效应。此外，THIP对乙醇相对于水消耗的不同影响表明，GABAA的调节可能在影响自愿乙醇饮用的获取中起作用。
