BERNHEIMER A W, CARLSON A S, KELLNER A
J Exp Med. 1956 Oct 1;104(4):577-87. doi: 10.1084/jem.104.4.577.
When solutions of streptolysin O were added to Warburg flasks containing, among other constituents, suspensions of mitochondria from the myocardium of rabbits and citrate, fumarate, or alpha-ketoglutarate as the substrate, there followed regularly a sharp reduction, and eventually complete cessation, of oxygen consumption. This phenomenon was not observed when succinate was the substrate in the flasks, the finding pointing to a selective interference with DPN as the underlying change. The agent in the solutions of streptolysin O responsible for this effect was shown to be a streptococcal product, and to be non-dialyzable and heat-labile. It differed from streptolysin O in that it did not appear to require prior activation with cysteine, and its effectiveness was not diminished by treatment with cholesterol or antistreptolysin globulins.
当将链球菌溶血素O溶液加入到含有兔心肌线粒体悬浮液以及柠檬酸盐、富马酸盐或α - 酮戊二酸作为底物等成分的瓦氏呼吸瓶中时，氧气消耗通常会急剧减少，最终完全停止。当琥珀酸盐作为呼吸瓶中的底物时，未观察到这种现象，这一发现表明潜在变化是对二磷酸吡啶核苷酸（DPN）的选择性干扰。链球菌溶血素O溶液中导致这种效应的物质被证明是一种链球菌产物，不可透析且对热不稳定。它与链球菌溶血素O的不同之处在于，它似乎不需要事先用半胱氨酸激活，并且用胆固醇或抗链球菌溶血素球蛋白处理不会降低其效力。
