Datla R S, Hammerlindl J K, Panchuk B, Pelcher L E, Keller W
Plant Biotechnology Institute, National Research Council of Canada, Saskatoon, Sask.
Gene. 1992 Dec 15;122(2):383-4. doi: 10.1016/0378-1119(92)90232-e.
The defective gene encoding neomycin phosphotransferase (NPTII) present in the binary plasmid vector, pBin19, was replaced with the wild-type (wt) gene. Plasmid vectors analogous to pBin19, pBI121 and pBI101 were constructed carrying the gene encoding the wt NPTII enzyme activity.
二元质粒载体pBin19中编码新霉素磷酸转移酶(NPTII)的缺陷基因被野生型(wt)基因取代。构建了与pBin19、pBI121和pBI101类似的质粒载体,这些载体携带编码野生型NPTII酶活性的基因。