甘蓝型油菜多聚半乳糖醛酸酶基因的启动子在转基因甘蓝型油菜植株中指导β-葡萄糖醛酸酶在花粉中的表达。
The promoter of aBrassica napus polygalacturonase gene directs pollen expression ofβ-glucuronidase in transgenicBrassica plants.
作者信息
Hong H P, Gerster J L, Datla R S S, Albani D, Scoles G, Keller W, Robert L S
机构信息
Plant Biotechnology Institute, National Research Council, 110 Gymnasium Road, S7N 0W9, Saskatoon, Saskatchewan, Canada.
Eastern Cereal and Oilseed Research Centre, Central Experimental Farm, K1A 0C6, Ottawa, Ontario, Canada.
出版信息
Plant Cell Rep. 1997 Mar;16(6):373-378. doi: 10.1007/BF01146776.
A 647-bp 5'-flanking fragment obtained from genomic clone Sta 44G(2) belonging to a family of polygalacturonase genes expressed inBrassica napus pollen was fused to theβ-glucuronidase (GUS) marker gene. This fusion construct was introduced intoB. napus plants viaAgrobacterium tumefaciens transformation. Analysis of the transgenicB. napus plants revealed that this promoter fragment is sufficient to direct GUS expression specifically in the anther and that GUS activity increases in pollen during maturation.
从属于在甘蓝型油菜花粉中表达的多聚半乳糖醛酸酶基因家族的基因组克隆Sta 44G(2)获得的一段647-bp 5'-侧翼片段，与β-葡萄糖醛酸酶（GUS）标记基因融合。该融合构建体通过根癌农杆菌转化导入甘蓝型油菜植株。对转基因甘蓝型油菜植株的分析表明，该启动子片段足以指导GUS仅在花药中特异性表达，并且在花粉成熟过程中GUS活性增加。
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