pBINPLUS：一种基于pBIN19的改良植物转化载体。
pBINPLUS: an improved plant transformation vector based on pBIN19.
作者信息
van Engelen F A, Molthoff J W, Conner A J, Nap J P, Pereira A, Stiekema W J
机构信息
Centre for Plant Breeding and Reproduction Research (CPRO-DLO), Department of Molecular Biology, Wageningen, The Netherlands.
出版信息
Transgenic Res. 1995 Jul;4(4):288-90. doi: 10.1007/BF01969123.
We describe the construction of a new plant transformation vector, pBINPLUS, based on the popular pBIN19 vector. Improvements over pBIN19 include location of the selectable marker gene at the left T-DNA border, a higher copy number in E. coli, and two rare restriction sites around the multiple cloning site for easier cloning and analysis of T-DNA insertions in plant genomes.
我们描述了一种基于常用的pBIN19载体构建的新型植物转化载体pBINPLUS。与pBIN19相比，改进之处包括：选择标记基因位于左T-DNA边界；在大肠杆菌中的拷贝数更高；多克隆位点周围有两个稀有酶切位点，便于在植物基因组中进行T-DNA插入的克隆和分析。
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