三苯基锍离子对线粒体的影响。三磷酸腺苷酶活性的抑制作用。
Effects of triphenylsulphonium ions on mitochondria. Inhibition of adenosine triphosphatase activity.
作者信息
Barrett R H, Selwyn M J
出版信息
Biochem J. 1976 May 15;156(2):315-22. doi: 10.1042/bj1560315.
Triphenylsulphonium ions inhibit mitochondrial oxidative phosphorylation and adenosine triphosphatase activity. The site of action is on the soluble F1 adenosine triphosphatase component. Triphenylsylphonium ions also inhibit electron transfer in the NAD-cytochrome b region of the respiratory chain. In both types of inhibition, triphenylsulphonium ions are effective at low concentrations, half-maximal inhibition being produced by a concentration of about 20-30 muM. These effects resemble the effects of alkylguanidines on mitochondria and are discussed in relation to the effects of alkylguanidines and other lipophilic cations such as ethidium and dibenzyldimethylammonium ions. A modification of the purification procedure for the soluble mitochondrial adenosine triphosphatase [Beechey, Hubbard, Linnett, Mitchell & Munn (1975) Biochem. J. 148, 533-537] IS DESCRIBED, WHICH YIELDS A PREPARATION WITH A HIGHER SPECIFIC ACTIVITY AND SHOWING FEWER BANDS IN GEL ELECTROPHORESIS.
三苯基锍离子可抑制线粒体氧化磷酸化及腺苷三磷酸酶活性。作用位点位于可溶性F1腺苷三磷酸酶组分上。三苯基锍离子还可抑制呼吸链中NAD - 细胞色素b区域的电子传递。在这两种抑制类型中，三苯基锍离子在低浓度时即有效，约20 - 30μM的浓度可产生半数最大抑制作用。这些效应类似于烷基胍对线粒体的效应，并结合烷基胍及其他亲脂性阳离子（如乙锭和二苄基二甲基铵离子）的效应进行了讨论。本文描述了一种可溶性线粒体腺苷三磷酸酶纯化方法的改进[Beechey、Hubbard、Linnett、Mitchell和Munn（1975年），《生物化学杂志》148卷，533 - 537页]，该方法得到的制剂具有更高的比活性，且在凝胶电泳中显示的条带更少。
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