Sato A, Sato Y, Suzuki A
Department of Autonomic Nervous System, Tokyo Metropolitan Institute of Gerontology, Japan.
Neurosci Res. 1992 Nov;15(3):189-98. doi: 10.1016/0168-0102(92)90004-v.
The effects of acupuncture-like stimulation of various segmental areas on the rhythmic micturition contractions (RMCs) of the urinary bladder were examined in anesthetized rats. The urinary bladder was cannulated via the urethra and expanded by infusing saline until the urinary bladder produced micturition contractions rhythmically as a consequence of the rhythmic burst discharges of the vesical pelvic efferent nerves. An acupuncture needle, having a diameter of either 160 or 340 microns, was inserted to a depth of about 4-5 mm into the skin and underlying muscles at various segmental areas, rostrally from the face then caudally to the hindlimb. Once being inserted, the needle was twisted left and right with the fingers about once every second for 1 min. (1) Acupuncture-like stimulation applied to the perineal area inhibited both the RMCs and the rhythmic burst discharges of vesical pelvic efferent nerves without any significant changes in the hypogastric efferent nerve activity. By contrast, stimulation applied to the face, neck, forelimb, chest, abdomen, back, and hindlimb areas was ineffective. (2) After surgically separating the perineal skin from the underlying muscles with the main cutaneous nerve branches intact, stimulation of either the perineal skin or the perineal muscles inhibited the RMCs. Stimulation of the perineal muscles produced a stronger inhibition of the RMCs than that of the perineal skin. (3) Stimulation of the perineal area increased afferent nerve activity, either recorded from the pudendal nerve branches innervating the perineal skin or underlying muscles, or recorded from the pelvic nerve branches innervating the perineal muscles. (4) The stimulation-induced inhibition of the RMCs was abolished after surgically severing both pudendal and pelvic nerve branches that innervated the perineal skin and underlying muscles. (5) The present findings indicate that the inhibition of the RMCs following acupuncture-like stimulation of the perineal area is a reflex response characterized by segmental organization. The afferent arcs of the reflex are both pelvic and pudendal nerve branches innervating the perineal skin and underlying muscles, while the efferent arcs are pelvic nerve branches innervating the urinary bladder.
在麻醉大鼠中，研究了针刺样刺激不同节段区域对膀胱节律性排尿收缩（RMCs）的影响。通过尿道将膀胱插管，并注入生理盐水使膀胱扩张，直到膀胱由于膀胱盆传出神经的节律性爆发放电而有节律地产生排尿收缩。将直径为160或340微米的针灸针插入从面部向前、然后向后肢尾侧的不同节段区域的皮肤及皮下肌肉中，深度约为4 - 5毫米。插入后，用手指左右捻转针，每秒约一次，持续1分钟。（1）针刺样刺激会阴区可抑制RMCs以及膀胱盆传出神经的节律性爆发放电，而腹下传出神经活动无明显变化。相比之下，刺激面部、颈部、前肢、胸部、腹部、背部和后肢区域则无效。（2）在手术分离会阴皮肤与皮下肌肉且主要皮神经分支完整后，刺激会阴皮肤或会阴肌肉均可抑制RMCs。刺激会阴肌肉对RMCs的抑制作用比刺激会阴皮肤更强。（3）刺激会阴区可增加传入神经活动，这些传入神经活动可从支配会阴皮肤或皮下肌肉的阴部神经分支记录到，也可从支配会阴肌肉的盆神经分支记录到。（4）在手术切断支配会阴皮肤和皮下肌肉的阴部神经和盆神经分支后，刺激诱导的RMCs抑制作用消失。（5）本研究结果表明，针刺样刺激会阴区后对RMCs的抑制是一种以节段性组织为特征的反射反应。该反射的传入弧是支配会阴皮肤和皮下肌肉的盆神经和阴部神经分支，而传出弧是支配膀胱的盆神经分支。
