Bergström T, Edström S, Tjellström A, Vahlne A
Department of Clinical Virology, University of Göteborg, Sahlgrens Hospital, Sweden.
Am J Otolaryngol. 1992 Sep-Oct;13(5):295-300. doi: 10.1016/0196-0709(92)90051-t.
It has been reported that the inner ear is capable of responding to antigen challenge. In this study, we have investigated the antibody reactivity to herpes simplex virus 1 (HSV-1) proteins in sera from patients with Ménière's disease.
Serum from 21 patients scheduled to undergo endolymphatic sac decompression for Ménière's disease was obtained. The sera from 21 age- and sex-matched individuals without a history of ear disease served as the control. An enzyme-linked immunosorbent assay (ELISA) was used to detect antibodies to HSV-1, HSV-2 cytomegalovirus (CMV), varicella-zoster virus (VZV), and measles virus. Immunoblotting was used to confirm and further evaluate the HSV-1 antibody response.
All but one patient had antibodies to HSV-1. ELISA mean log titers were significantly lower in the patient group to CMV and VZV when compared with controls. A pattern of generally higher antibody reactivity in patients with Ménière's disease was demonstrated to the individual SDS-PAGE HSV-1 polypeptides as judged by immunoblotting.
The HSV-1 antibody response found in patients with Ménière's disease may indicate viral reactivation and denotes the importance of further studies on the role of infectious agents in this disease.
据报道，内耳能够对抗原刺激作出反应。在本研究中，我们调查了梅尼埃病患者血清中对单纯疱疹病毒1型（HSV-1）蛋白的抗体反应性。
获取了21例计划接受梅尼埃病内淋巴囊减压术患者的血清。21名年龄和性别匹配且无耳部疾病史的个体的血清作为对照。采用酶联免疫吸附测定（ELISA）检测针对HSV-1、HSV-2、巨细胞病毒（CMV）、水痘带状疱疹病毒（VZV）和麻疹病毒的抗体。免疫印迹法用于确认并进一步评估HSV-1抗体反应。
除1例患者外，所有患者均有HSV-1抗体。与对照组相比，患者组针对CMV和VZV的ELISA平均对数滴度显著较低。通过免疫印迹法判断，梅尼埃病患者对单个十二烷基硫酸钠聚丙烯酰胺凝胶电泳（SDS-PAGE）HSV-1多肽的抗体反应性总体上更高。
梅尼埃病患者中发现的HSV-1抗体反应可能表明病毒再激活，并表明进一步研究感染因子在该疾病中的作用具有重要意义。
