水痘-带状疱疹病毒糖蛋白3（VZV gp3）诱导针对水痘-带状疱疹病毒（VZV）的中和抗体以及VZV gp3与单纯疱疹病毒糖蛋白B（gB）之间的交叉反应性

Induction of neutralizing antibody against varicella-zoster virus (VZV) by VZV gp3 and cross-reactivity between VZV gp3 and herpes simplex viruses gB.

作者信息

Kitamura K, Namazue J, Campo-Vera H, Ogino T, Yamanishi K

出版信息

Virology. 1986 Feb;149(1):74-82. doi: 10.1016/0042-6822(86)90088-7.

DOI:10.1016/0042-6822(86)90088-7

Abstract

Glycoprotein gp3 (64K) is one of the major proteins specified by varicella-zoster virus (VZV). This glycoprotein was purified on an immunoadsorbent consisting of monoclonal antibody (clone 8) against gp3 linked to protein A-Sepharose. Rabbits were then immunized with the purified antigen to obtain monospecific antisera against gp3. The monospecific antisera and monoclonal antibody immunoprecipitated polypeptides with the same molecular weights of approximately 64,000 (64K), 106K, and 116K from a lysate of labeled cells infected with VZV. The monoclonal antibodies against gp3 did not have neutralizing activity against VZV, but anti-gp3 monospecific sera neutralized VZV infectivity. The antigenic relation of VZV to herpes simplex virus (HSV) was investigated by the immunofluorescent test, immunoprecipitation followed by analysis by sodium dodecyl sulfate-polyacrylamide gel electrophoresis and the neutralizing antibody test with monoclonal antibodies and monospecific antisera. In the indirect immunofluorescent test, the cytoplasm of cells infected with HSV-type 1 or HSV-type 2 was stained with anti-gp3 monospecific antiserum but not with anti-gp3 monoclonal antibodies. This serum also precipitated the polypeptides of HSV-type 1 and HSV-type 2 with molecular weight of approximately 120,000, possibly corresponding to gB of HSV-1 or HSV-2, and this immunoprecipitation was blocked by anti-gB monoclonal antibody. However, anti-gp3 monospecific antisera did not neutralize either HSV-type 1 or HSV-type 2 infectivity. These results suggest that gp3 induces neutralizing antibody against VZV and that it also has a cross-reacting antigenic determinant with gB of HSV.

摘要

糖蛋白gp3（64K）是水痘-带状疱疹病毒（VZV）特有的主要蛋白之一。该糖蛋白在由与蛋白A-琼脂糖偶联的抗gp3单克隆抗体（克隆8）组成的免疫吸附剂上进行纯化。然后用纯化的抗原免疫兔子，以获得抗gp3的单特异性抗血清。该单特异性抗血清和单克隆抗体从感染VZV的标记细胞裂解物中免疫沉淀出分子量约为64,000（64K）、106K和116K的相同多肽。抗gp3单克隆抗体对VZV没有中和活性，但抗gp3单特异性血清可中和VZV的感染性。通过免疫荧光试验、免疫沉淀后进行十二烷基硫酸钠-聚丙烯酰胺凝胶电泳分析以及用单克隆抗体和单特异性抗血清进行中和抗体试验，研究了VZV与单纯疱疹病毒（HSV）的抗原关系。在间接免疫荧光试验中，感染1型或2型HSV的细胞胞质被抗gp3单特异性抗血清染色，但未被抗gp3单克隆抗体染色。该血清还沉淀出分子量约为120,000的1型和2型HSV多肽，可能对应于HSV-1或HSV-2的gB，并且这种免疫沉淀被抗gB单克隆抗体阻断。然而，抗gp3单特异性抗血清不能中和1型或2型HSV的感染性。这些结果表明，gp3可诱导针对VZV的中和抗体，并且它还与HSV的gB具有交叉反应性抗原决定簇。