Dimitriadis V K, Nikolaidis K, Sivropoulou A
Department of Genetics, Development and Molecular Biology, School of Biology, Aristotle University of Thessaloniki, Greece.
Microbios. 1992;72(291):119-27.
Baby hamster kidney (BHK tk-) cells infected with herpes simplex virus-1 (HSV-1) showed a large number of virus particles isolated in vesicles characterized by the presence or the absence of ribosomes or inside cisternae of the rough endoplasmic reticulum or the nuclear envelope. The isolation of the virions by intracellular membranes appeared shortly after infection of the cells by HSV-1. These structures persisted for longer periods where no morphological alterations in the infected cells were noted as well as at periods where expression of the late viral genes and the presence of empty capsids or DNA-containing new capsids in the nucleoplasm of BHK tk- cells were detected. The results suggest that the presence of virions in membranic formations of the infected cells may be an indication of permanent isolation and subsequent deactivation of the viruses rather than an intermediate stage during their transport from the plasma membrane to the nucleus. The possible mechanisms by which the virions are isolated by the intracellular membranes of BHK tk- cells are discussed.
感染单纯疱疹病毒1型（HSV-1）的幼仓鼠肾（BHK tk-）细胞显示，大量病毒颗粒在囊泡中分离出来，这些囊泡的特征是存在或不存在核糖体，或位于粗面内质网或核膜的池内。在HSV-1感染细胞后不久，细胞内膜就出现了病毒粒子的分离。这些结构持续了较长时间，在此期间，未观察到感染细胞有形态学改变，同时也检测到了BHK tk-细胞核质中晚期病毒基因的表达以及空衣壳或含DNA的新衣壳的存在。结果表明，感染细胞的膜性结构中存在病毒粒子可能表明病毒被永久隔离并随后失活，而不是病毒从质膜运输到细胞核过程中的一个中间阶段。本文讨论了BHK tk-细胞内膜隔离病毒粒子的可能机制。
