Dargan D J, Subak-Sharpe J H
M.R.C. Virology Unit, Division of Virology, Institute of Biomedical and Life Sciences, University of Glasgow, United Kingdom.
Virology. 1997 Dec 22;239(2):378-88. doi: 10.1006/viro.1997.8893.
Herpes simplex virus type 1(HSV-1) L-particles are known to be composed mainly of envelope and tegument proteins, to lack the nucleocapsid, and to be noninfectious. Thus L-particles represent interesting vaccine candidates. L-particles at > 1000/cell interfered with HSV-1 virion adsorption and penetration While L-particles did not affect HSV-1 growth kinetics in resting or nonresting BHK cultures infected with purified virions, treatment with L-particles before, or after, transfection with HSV-1 DNA resulted in a progressive increase in plaque numbers (five- to sixfold at 1000 L-particles/cell). Transfection assays using HSV-1 ts mutant DNA (ts 1201) revealed that enhancement was due to induction of otherwise nonreplicating genomes. The enhancement obtained with L-particles produced by WT HSV-1 or by mutants that are either deleted, or defective, in certain gene products was compared. Most important were the Vmw110 (ICP0) and Vmw65 (alpha-TIF) proteins, but VP11/12, VP13/14, and vhs also have a role. The L-particle-associated Vmw175 (ICP 4) protein did not appear be involved. The effect of homologous and heterologous combinations of pseudorabies virus, equineherpesvirus-1, and HSV-1 DNA's and L-particles was investigated in transfection assays. The L-particles of each virus, to varying extent, enhanced the plaquing efficiency of their own DNA but were also effective in heterologous combinations.
已知单纯疱疹病毒1型（HSV-1）L颗粒主要由包膜和被膜蛋白组成，缺乏核衣壳，且无感染性。因此，L颗粒是有趣的疫苗候选物。每细胞>1000个L颗粒会干扰HSV-1病毒体的吸附和穿透。虽然L颗粒不影响用纯化病毒体感染的静止或非静止BHK培养物中HSV-1的生长动力学，但在用HSV-1 DNA转染之前或之后用L颗粒处理会导致噬斑数量逐渐增加（每细胞1000个L颗粒时增加五到六倍）。使用HSV-1温度敏感突变体DNA（ts1201）进行的转染试验表明，增强作用是由于诱导了原本不复制的基因组。比较了野生型HSV-1或某些基因产物缺失或有缺陷的突变体产生的L颗粒所获得的增强作用。最重要的是Vmw110（ICP0）和Vmw65（α-TIF）蛋白，但VP11/12、VP13/14和vhs也有作用。与L颗粒相关的Vmw175（ICP 4）蛋白似乎未参与其中。在转染试验中研究了伪狂犬病病毒、马疱疹病毒1型和HSV-1 DNA与L颗粒的同源和异源组合的作用。每种病毒的L颗粒在不同程度上提高了其自身DNA的噬斑形成效率，但在异源组合中也有效。
