Chow K C, Nacilla J Q, Witzig T E, Li C Y
Department of Laboratory Medicine and Pathology, Mayo Clinic, Rochester, Minnesota 55905.
Am J Hematol. 1992 Dec;41(4):270-5. doi: 10.1002/ajh.2830410409.
Previous reports of patients with persistent polyclonal B lymphocytosis have found associations with female sex, cigarette smoking, HLA-DR7 phenotype, and moderate elevation of peripheral blood polyclonal B lymphocytes. The presence of binucleated cells and atypical lymphocytes in the peripheral blood of these patients was highly suggestive of a viral infection, such as with the Epstein-Barr virus. We report a 47-year-old asymptomatic woman who was incidentally found to have persistent polyclonal B lymphocytosis and serum IgG against virus capsid antigen (VCA) and Epstein-Barr virus (EBV)-associated nuclear antigen (EBNA) of EBV. The presence of EBV was investigated in the peripheral blood lymphocytes from this patient by in situ hybridization and polymerase chain reaction methods. EBV DNA was demonstrated in the lymphocyte fraction by polymerase chain reaction, and it was further located in lymphoid cells by in situ hybridization. These results indicate that persistent polyclonal B lymphocytosis is strongly associated with EBV.
先前关于持续性多克隆B淋巴细胞增多症患者的报道发现，该病与女性、吸烟、HLA - DR7表型以及外周血多克隆B淋巴细胞中度升高有关。这些患者外周血中出现双核细胞和非典型淋巴细胞，强烈提示存在病毒感染，如感染爱泼斯坦 - 巴尔病毒（EBV）。我们报告了一名47岁无症状女性，她偶然被发现患有持续性多克隆B淋巴细胞增多症，并且血清中存在针对EBV病毒衣壳抗原（VCA）和EBV相关核抗原（EBNA）的IgG。通过原位杂交和聚合酶链反应方法对该患者外周血淋巴细胞中的EBV进行了检测。通过聚合酶链反应在淋巴细胞组分中检测到EBV DNA，并且通过原位杂交进一步将其定位在淋巴样细胞中。这些结果表明，持续性多克隆B淋巴细胞增多症与EBV密切相关。
