Trefzger J, Ronai A, Wassmer B, von Deimling O
Abteilung für Chemische Pathologie, Universität Freiburg, Germany.
Histochemistry. 1992 Dec;98(6):373-9. doi: 10.1007/BF00271073.
After inhibition by bis-p-nitrophenyl phosphate and subsequent staining for esterase using naphthol AS-D acetate as the substrate, a strong lysosomal esterase was demonstrated in the cauda epididymidis of mouse, rat, and man. Owing to its behaviour towards the classifying inhibitors eserine, diisopropyl fluorophosphate, bis-p-nitrophenyl phosphate, and p-chloromercuriphenylsulphonate, this lysosomal esterase was shown to be an acetylesterase (EC 3.1.1.6). Control experiments involving isoelectric focusing revealed that this acetylesterase was identical with the genetically defined homologues ES-17, ES-6, and ES-A4 in mouse, rat, and man, respectively.
在用对硝基苯磷酸双酯抑制后，随后以萘酚AS - D醋酸酯为底物进行酯酶染色，在小鼠、大鼠和人的附睾尾部均显示出强溶酶体酯酶。由于其对分类抑制剂毒扁豆碱、二异丙基氟磷酸酯、对硝基苯磷酸双酯和对氯汞苯磺酸盐的反应，这种溶酶体酯酶被证明是一种乙酰酯酶（EC 3.1.1.6）。涉及等电聚焦的对照实验表明，这种乙酰酯酶分别与小鼠、大鼠和人中基因定义的同系物ES - 17、ES - 6和ES - A4相同。
