PAPPENHEIMER A M, SALVIN S B, UHR J W
J Exp Med. 1957 Jan 1;105(1):11-24. doi: 10.1084/jem.105.1.11.
A general method for induction of the delayed hypersensitive state directed against single protein antigens is described. The method consists of intradermal injection of minute amounts of washed immune precipitates containing the antigen in question. Provided the specific precipitates are formed in the region of antibody excess, maximal sensitivity develops at least 2 to 3 weeks before detectable circulating antibody is formed in guinea pigs against the sensitizing antigen. Neither adjuvant nor killed acid-fast bacteria are required for induction of the delayed hypersensitive state although the degree of sensitization is considerably increased when the sensitizing material is incorporated in Freund's complete adjuvant. Characteristics of the "delayed" as opposed to the "immediate" hypersensitive states in the guinea pig are described and implications of the findings are discussed.
本文描述了一种针对单一蛋白质抗原诱导迟发型超敏反应状态的通用方法。该方法包括皮内注射微量含有相关抗原的洗涤过的免疫沉淀物。如果特异性沉淀物在抗体过量区域形成,在豚鼠体内针对致敏抗原形成可检测到的循环抗体之前至少2至3周,会产生最大敏感性。诱导迟发型超敏反应状态既不需要佐剂也不需要灭活的抗酸菌,不过当致敏物质与弗氏完全佐剂混合时,致敏程度会显著增加。文中描述了豚鼠中“迟发”与“速发”超敏反应状态的特征,并讨论了这些发现的意义。