SHEPARD C C
J Exp Med. 1957 Jan 1;105(1):39-48. doi: 10.1084/jem.105.1.39.
By making use of the increased phagocytosis which follows the exposure of HeLa cells to tissue culture media containing selected horse sera, it was possible to introduce all of the mycobacterial species studied into the cells, where many of them proceeded to grow. Fully virulent strains of tubercle bacilli filled much of the cytoplasm in a few days and formed characteristic cords not seen with other strains. The strains said to be less virulent, R1Rv, BCG, H37Ra, and R1Ra, grew less rapidly and in characteristic patterns. Their rates of multiplication in HeLa cells were in the order named and correlated well with their reported pathogenicity for mice and guinea pigs. Six INH-resistant strains grew at rates characteristic of fully virulent strains. Among the "rapidly growing" species, M. phlei and M. smegmatis did not show evidence of growth in the cells, although M. fortuitum did. Some strains with optimal temperatures on bacteriological media below 37 degrees C, M. balnei, M. marinum, and M. platypoecilus, grew rapidly in HeLa cells, especially at temperatures of 31 to 35 degrees C. The growth patterns of the bacilli in HeLa cells appear sufficiently specific to be useful in differentiation among the mycobacteria.
通过利用将HeLa细胞暴露于含有特定马血清的组织培养基后增强的吞噬作用，有可能将所有研究的分枝杆菌种类引入细胞中，其中许多种类随后开始生长。完全有毒力的结核杆菌菌株在几天内充满了大部分细胞质，并形成了其他菌株未见的特征性索状结构。据说毒力较低的菌株，如R1Rv、卡介苗（BCG）、H37Ra和R1Ra，生长较慢且具有特征性模式。它们在HeLa细胞中的增殖速率按上述顺序排列，并且与它们对小鼠和豚鼠的报道致病性密切相关。六种耐异烟肼菌株以完全有毒力菌株的特征速率生长。在“快速生长”的种类中，草分枝杆菌和耻垢分枝杆菌在细胞中未显示生长迹象，尽管偶然分枝杆菌显示有生长。一些在低于37摄氏度的细菌培养基上具有最佳温度的菌株，如海鱼分枝杆菌、海分枝杆菌和平胸分枝杆菌，在HeLa细胞中生长迅速，尤其是在31至35摄氏度的温度下。分枝杆菌在HeLa细胞中的生长模式似乎足够特异，可用于分枝杆菌之间的鉴别。
