免疫球蛋白A肾病中的人巨细胞病毒：通过聚合酶链反应检测

Human cytomegalovirus in immunoglobulin A nephropathy: detection by polymerase chain reaction.

作者信息

Müller G A, Müller C A, Engler-Blum G, Kühn W, Risler T, Bohle A, Markovic-Lipkovski J

机构信息

Department 3, Eberhard-Karls University, Tübingen, FRG.

出版信息

Nephron. 1992;62(4):389-93. doi: 10.1159/000187086.

DOI:10.1159/000187086

PMID:1338641

Abstract

Human cytomegalovirus (HCMV) has been suspected to participate in the pathogenesis of IgA nephropathy (IgAN). However, with regard to the presence of HCMV in the renal tissue of IgAN, conflicting results have been reported using a variety of different techniques. Renal biopsies of 29 patients with IgAN, of 7 with focal segmental glomerulosclerosis (FSGS) and of 11 normal kidneys were analyzed for the presence of HCMV-DNA using the polymerase chain reaction. HCMV-DNA was detected by hybridization with digoxigenin-labelled probes in 14 of 19 analyzed frozen renal biopsies from patients with IgAN. However, only 1 of 17 renal biopsies of IgAN embedded in paraffin was positive for HCMV-DNA. Furthermore, HCMV-DNA was detected in 4 of 18 frozen normal kidneys but in none of the tissues from patients with FSGS. The present results provide further evidence of an association between the presence of HCMV in renal tissue and IgAN.

摘要

人巨细胞病毒（HCMV）被怀疑参与IgA肾病（IgAN）的发病机制。然而，关于IgAN肾组织中HCMV的存在情况，使用各种不同技术报告的结果相互矛盾。采用聚合酶链反应分析了29例IgAN患者、7例局灶节段性肾小球硬化（FSGS）患者和11例正常肾脏的肾活检组织中HCMV-DNA的存在情况。通过与地高辛标记探针杂交，在19例分析的IgAN患者冰冻肾活检组织中有14例检测到HCMV-DNA。然而，在17例石蜡包埋的IgAN肾活检组织中只有1例HCMV-DNA呈阳性。此外，在18例冰冻正常肾脏中有4例检测到HCMV-DNA，但FSGS患者的组织中均未检测到。目前的结果进一步证明了肾组织中HCMV的存在与IgAN之间的关联。