Gerna G, Baldanti F, Zella D, Furione M
Viral Diagnostic Service, IRCCS Policlinico San Matteo, University of Pavia, Italy.
Scand J Infect Dis Suppl. 1995;99:11-5.
Although several methods have been utilized for the detection and quantification of human cytomegalovirus (HCMV) DNA, all of them can be divided into three groups: (i) detection of HCMV DNA directly in tissues by in situ hybridization or in situ polymerase chain reaction (PCR); (ii) detection of HCMV DNA in cell or tissue lysates by hybridization with DNA or RNA probes differently labelled-labels were progressively modified in order to provide an increasing sensitivity (hybridization products were revealed by radioactive, colorimetric or chemiluminescent procedures); (iii) detection of HCMV DNA in cell or tissue lysates by qualitative (single-step and nested) and quantitative (semiquantitative, competitive or noncompetitive) PCR. The selection of the methods to be employed depends primarily on the clinical situation which must be evaluated. Clinical samples for HCMV genome detection must vary accordingly.
虽然已采用多种方法检测和定量人巨细胞病毒（HCMV）DNA，但所有这些方法可分为三类：（i）通过原位杂交或原位聚合酶链反应（PCR）直接在组织中检测HCMV DNA；（ii）通过与不同标记的DNA或RNA探针杂交来检测细胞或组织裂解物中的HCMV DNA——标记不断改进以提高灵敏度（杂交产物通过放射性、比色或化学发光程序显示）；（iii）通过定性（单步和巢式）和定量（半定量、竞争性或非竞争性）PCR检测细胞或组织裂解物中的HCMV DNA。所采用方法的选择主要取决于必须评估的临床情况。用于HCMV基因组检测的临床样本必须相应地有所不同。
