Wild C P, Hudson G J, Sabbioni G, Chapot B, Hall A J, Wogan G N, Whittle H, Montesano R, Groopman J D
International Agency for Research on Cancer, Lyon, France.
Cancer Epidemiol Biomarkers Prev. 1992 Mar-Apr;1(3):229-34.
Aflatoxin is implicated as a risk factor for hepatocellular carcinoma in areas of the world with a high incidence of this tumor. The present study was designed to validate the use of aflatoxin-albumin adducts in peripheral blood as a measure of individual exposure to this carcinogen. Dietary intake of aflatoxin was measured at the individual level in 20 residents of Keneba, West Kiang, The Gambia, over a 7-day period and correlated with the level of aflatoxin bound to peripheral blood albumin at the beginning and end of the study. Complementary enzyme-linked immunosorbent assay and high-performance liquid chromatography-fluorescence techniques were used to assay the aflatoxin adducts. All subjects were exposed to aflatoxin originating from several food types, with an average daily intake of 1.4 micrograms/day. A significant correlation (r = 0.55; P = < 0.05) was observed between the dietary intake and the level of albumin-bound aflatoxin at the end of the study. In addition, a good agreement was obtained with the two analytical techniques. A comparison of matched chronic hepatitis B surface antigen carriers with noncarriers did not reveal any difference in adduct formation for a given dietary intake of aflatoxin. These studies demonstrate the validity of aflatoxin-albumin adducts as a marker of human exposure to this carcinogen.
在世界上肝细胞癌高发地区，黄曲霉毒素被认为是该病的一个风险因素。本研究旨在验证外周血中黄曲霉毒素 - 白蛋白加合物作为个体接触这种致癌物的一种衡量指标的有效性。在冈比亚西江区凯内巴的20名居民中，对其7天内的黄曲霉毒素膳食摄入量进行个体水平测量，并将其与研究开始和结束时外周血白蛋白结合的黄曲霉毒素水平相关联。采用互补的酶联免疫吸附测定法和高效液相色谱 - 荧光技术检测黄曲霉毒素加合物。所有受试者均接触源自多种食物类型的黄曲霉毒素，平均每日摄入量为1.4微克/天。研究结束时，观察到膳食摄入量与白蛋白结合的黄曲霉毒素水平之间存在显著相关性（r = 0.55；P = < 0.05）。此外，两种分析技术之间具有良好的一致性。对于给定的黄曲霉毒素膳食摄入量，比较配对的慢性乙型肝炎表面抗原携带者和非携带者，未发现加合物形成有任何差异。这些研究证明了黄曲霉毒素 - 白蛋白加合物作为人类接触这种致癌物的标志物的有效性。
