Oldham R K, Dean J H, Cannon G B, Ortaldo J R, Dunston G, Applebaum F, McCoy J L, Djeu J, Herberman R B
Int J Cancer. 1976 Aug 15;18(2):145-55. doi: 10.1002/ijc.2910180203.
Methods are described by which cryopreserved cells can be utilized in a number of in vitro assays. On a per cell basis, nearly total recovery of function can be demonstrated for lymphocyte transformation (mitogens, antigens and MLC) and rosette-forming cells. Excellent recovery of mononuclear cell production of leukocyte migration inhibitory factor was also observed. Highly reproducible activity was recovered on a per lymphocyte basis in lymphocyte cytotoxicity but with a definite decrement in the percentage recovery. Both for longitudinal studies of immune function and for standardization of these assays in one or more laboratories such cryopreserved cells are ofimmense value and should be widely utilized.
本文描述了一些方法，通过这些方法可将冷冻保存的细胞用于多种体外测定。以单个细胞为基础，在淋巴细胞转化（有丝分裂原、抗原和混合淋巴细胞培养）和玫瑰花结形成细胞方面，几乎可证明功能的完全恢复。还观察到单核细胞产生白细胞迁移抑制因子的回收率极佳。在淋巴细胞细胞毒性试验中，以单个淋巴细胞为基础可回收高度可重复的活性，但回收率的百分比有一定程度的下降。对于免疫功能的纵向研究以及在一个或多个实验室中对这些测定进行标准化而言，此类冷冻保存的细胞具有巨大价值，应得到广泛应用。
