Pross H F, Baines M G, Rubin P, Shragge P, Patterson M S
J Clin Immunol. 1981 Jan;1(1):51-63. doi: 10.1007/BF00915477.
On analysis of in vitro assays of human natural killer (NK) cell function the inadequacy of commonly used methods of expressing lytic activity was apparent. A comparison was made of the data obtained using modifications of two equations-the simple exponential fit and the von Krogh equations. Both of these equations were found to satisfy the following essential criteria for use in these assays. First, the majority of the results obtained in the chromium-release assay could be used in data reduction; second, the resultant "dose-response" curve was reduced to linearity; and third, a single numerical expression was obtained which was directly proportional to the cytotoxic activity. Of the two methods the more conventional exponential fit was found to be the simpler to use. The closeness of fit of the experimentally derived data to the ideal curves did not support the possibility that normal lymphocyte preparations contain suppressor cells capable of inhibiting NK activity. Data have also been presented showing that NK-sensitive targets could be categorized with respect to their susceptibility by comparing the slopes of the target cell survival curves obtained using the exponential fit equation. These observations are relevant to the accurate assessment of NK activity in patient populations and to the determination of the effects of disease and its treatment on this activity.
在对人类自然杀伤（NK）细胞功能的体外试验分析中，常用的表达溶解活性方法的不足之处显而易见。对使用两个方程（简单指数拟合和冯·克罗伊方程）的修改所获得的数据进行了比较。发现这两个方程均满足这些试验使用的以下基本标准。首先，在铬释放试验中获得的大多数结果可用于数据简化；其次，所得的“剂量反应”曲线简化为线性；第三，获得了一个与细胞毒性活性成正比的单一数值表达式。在这两种方法中，发现更传统的指数拟合使用起来更简单。实验得出的数据与理想曲线的拟合紧密程度不支持正常淋巴细胞制剂含有能够抑制NK活性的抑制细胞的可能性。还提供了数据表明，通过比较使用指数拟合方程获得的靶细胞存活曲线的斜率，可以根据NK敏感靶标的敏感性对其进行分类。这些观察结果与准确评估患者群体中的NK活性以及确定疾病及其治疗对该活性的影响有关。
