Yamada K, Ukai M
Histochemistry. 1976 Jun 28;47(3):219-38. doi: 10.1007/BF00489964.
In order to study the histochemical nature of mucosaccharides in germfree animals, the organs in natural contact with bacteria (stomach, small and large intestine) and those naturally remote from bacteria (tracheal and ear cartilage and aorta) were studied by means of light microscopic methods for mucosaccharides in germfree and conventional rats. In the stomach (surface and foveolar cells) of germfree rats the histochemical reactions for acid and neutral mucosaccharides were apparently less intense than in that of conventional rats, whereas in the small and large intestine (goblet cells) of germfree rats the reactions were significantly more intense than in those of conventional rats. In the cartilage (intercellular matrix, lacunar border and chondrocyte cytoplasm) and aorta (interelastic spaces) of germfree animals the reactions were less intense than in those of conventional animals. In addition, some differences in the histochemical nature of mucosaccharides between the organs of germfree and conventional rats were noted, as revealed by the effects of chemical modifications and digestions with enzymes upon the histochemical reactions studied.
为了研究无菌动物中粘多糖的组织化学性质，采用光学显微镜方法，对无菌大鼠和普通大鼠中与细菌自然接触的器官（胃、小肠和大肠）以及自然远离细菌的器官（气管、耳软骨和主动脉）中的粘多糖进行了研究。在无菌大鼠的胃（表面和小凹细胞）中，酸性和中性粘多糖的组织化学反应明显不如普通大鼠强烈，而在无菌大鼠的小肠和大肠（杯状细胞）中，反应明显比普通大鼠强烈。在无菌动物的软骨（细胞间基质、腔隙边界和软骨细胞胞质）和主动脉（弹性纤维间隙）中，反应不如普通动物强烈。此外，通过化学修饰和酶消化对所研究的组织化学反应的影响发现，无菌大鼠和普通大鼠器官之间粘多糖的组织化学性质存在一些差异。
