Díaz-Guerra O, Rico G, Ladrón de Guevara O, Padilla P, García L, Kretschmer R R
División de Inmunología, Subjefatura de Investigación, CMN Siglo XXI, IMSS, México, DF.
Arch Med Res. 1992;23(2):151-2.
Ultrafiltration, gel-sieve chromatography and HPLC were used to purify MLIF. This material was HCl-hydrolyzed and the amino acids analyzed by HPLC-ortho-ophthaldialdehyde. Two hydrophobic non-polar (ile,leu), two uncharged polar (thr,tyr) and two positively charged (basic) (his, arg) amino acids were found in clear excess to their concentration in AMC and thus may intervene in the composition of MLIF.