Linder E, Lundin L, Haglund S, Hallander H, Tellez Sierra A
National Bacteriological Laboratory, Stockholm, Sweden.
Arch Med Res. 1992;23(2):169-72.
To avoid false positive reactions in tests for anti-ameba antibodies, we wanted to identify parasite-specific component(s). Amebiasis patient sera recognized an antigen of 67 kDa by immunoblotting in an active E. histolytica fraction obtained by ion exchange chromatography. Monoclonal antibodies against the fraction were made. Antibody 3G2 reacted with three antigenic components of 67, 40 and 25 kDa and in the immunocytology with an epitope located in the cytoplasm of E. histolytica trophozoites. ELISAs using the isolated parasite fraction and monoclonal antibody 3G2 (to assay inhibition of binding) were capable of distinguishing specific reactivity in sera from amebiasis patients.
为避免抗阿米巴抗体检测中出现假阳性反应，我们希望鉴定寄生虫特异性成分。阿米巴病患者血清通过免疫印迹法在经离子交换色谱法获得的活性溶组织内阿米巴组分中识别出一种67 kDa的抗原。制备了针对该组分的单克隆抗体。抗体3G2与67、40和25 kDa的三种抗原成分发生反应，并且在免疫细胞学中与位于溶组织内阿米巴滋养体细胞质中的一个表位发生反应。使用分离出的寄生虫组分和单克隆抗体3G2的ELISA（用于检测结合抑制）能够区分阿米巴病患者血清中的特异性反应性。
