溶组织内阿米巴组蛋白H3的cDNA克隆

cDNA cloning of Entamoeba histolytica histone H3.

作者信息

Födinger M, Ortner S, Plaimauer B, Wiedermann G, Scheiner O, Duchêne M

机构信息

Institute of Specific Prophylaxis and Tropical Medicine, University of Vienna, Austria.

出版信息

Arch Med Res. 1992;23(2):19-21.

PMID:1340291

Abstract

E. histolytica has an unusual nuclear structure. Although nucleosome-like particles were observed, no information about histones was available so far. In this paper we describe a cDNA clone with significant homology to H3 histones that was isolated from a lambda Zap library of pathogenic E. histolytica. The complete cDNA encodes a 15 kDa polypeptide, which is shorter by one residue than the human homologue. The amino acid sequence has only 69% identity with human H3.3 histone. Our results indicate that this divergence may contribute to the unusual chromatin structure of E. histolytica.

摘要

溶组织内阿米巴具有独特的核结构。尽管观察到了核小体样颗粒，但迄今为止尚无关于组蛋白的信息。在本文中，我们描述了一个与H3组蛋白具有显著同源性的cDNA克隆，该克隆是从致病性溶组织内阿米巴的λZap文库中分离出来的。完整的cDNA编码一个15 kDa的多肽，比人类同源物少一个残基。该氨基酸序列与人类H3.3组蛋白的同一性仅为69%。我们的结果表明，这种差异可能导致了溶组织内阿米巴异常的染色质结构。