Tanaka T, Tanaka M, Mitsui Y
Division of Host Defense Mechanism, Tokai University School of Medicine, Isehara, Kanagawa, Japan.
Biochem Biophys Res Commun. 1997 Jul 30;236(3):611-5. doi: 10.1006/bbrc.1997.7019.
A directional cDNA library constructed from mRNA of the trophozoite of Entamoeba histolytica HM-1:IMSS strain was used for the generation of expressed sequence tags (ESTs). From 5' ends of the distinct cDNA clones, 105 ESTs were obtained. Of these, 30 clones (29%) were previously known E. histolytica genes. Forty-five clones (42%) had matches with entries for other organisms in the databases. These new E. histolytica genes constituted a broad range of transcripts distributed among cytoplasmic structural and regulatory proteins, enzymes, nuclear and other proteins, and proteins of unknown function. Thirty clones (29%) had no significant database matches and thus potentially represent E. histolytica-specific genes. These data of E. histolytica genes identified by nucleotide sequencing indicate the value of the adoption of genome sequencing strategies for the rapid increase in knowledge of organisms causing dysentery and liver abscess.
从溶组织内阿米巴HM-1:IMSS株滋养体的mRNA构建的定向cDNA文库用于生成表达序列标签（EST）。从不同cDNA克隆的5'端获得了105个EST。其中，30个克隆（29%）是先前已知的溶组织内阿米巴基因。45个克隆（42%）与数据库中其他生物体的条目相匹配。这些新的溶组织内阿米巴基因构成了广泛的转录本，分布在细胞质结构和调节蛋白、酶、核蛋白和其他蛋白以及功能未知的蛋白中。30个克隆（29%）在数据库中没有显著匹配，因此可能代表溶组织内阿米巴特异性基因。通过核苷酸测序鉴定的这些溶组织内阿米巴基因数据表明，采用基因组测序策略对于快速增加对引起痢疾和肝脓肿的生物体的了解具有重要价值。
