Rangel A, Mora N, Espinoza A, Cristancho M, Hernández M
Departamento de Medicina, Facultad de Medicina, Universidad de Los Andes, Mérida, Venezuela.
Arch Med Res. 1992;23(2):259-60.
The antigens of E. histolytica recognized by IgA antibodies in sera from patients with either amebic liver abscess or intestinal amebiasis, as well as saliva from the latter, were determined in immunoblots of whole native trophozoite proteins. Results show that sera of patients with amebic liver abscess reacted mainly with amebic proteins of more than 200 kDa, whereas those of individuals with intestinal amebiasis recognized a protein of 145 kDa. The saliva of the latter detected a protein of 210 kDa which is not always seen when using the sera of patients with intestinal or hepatic amebiasis.
通过对完整天然滋养体蛋白进行免疫印迹分析，确定了阿米巴肝脓肿患者或肠道阿米巴病患者血清以及后者唾液中被IgA抗体识别的溶组织内阿米巴抗原。结果显示，阿米巴肝脓肿患者的血清主要与分子量超过200 kDa的阿米巴蛋白发生反应，而肠道阿米巴病患者的血清识别出一种分子量为145 kDa的蛋白。后者的唾液检测到一种分子量为210 kDa的蛋白，而在使用肠道或肝阿米巴病患者的血清时并不总是能检测到该蛋白。
