Berber A C, Escobar A, Zamora M, Acosta G
Unidad de Inve., Escuela Superior de Medicina, IPN, México, D.F.
Arch Invest Med (Mex). 1990;21 Suppl 1:97-101.
Immunodetection of total antigens of Entamoeba histolytica trophozoites transferred to nitrocellulose paper was realized with IgA antibodies present in colostra and sera samples of 28 puerperal women and in 24 sera samples of patients with a diagnosis of amebic hepatic abscess. The most frequently recognized antigens by colostra antibodies were those which correspond to 1290, 82.79, 52.14, 40.91, 37.75, 35.08, 33.52, 28.53, 27.53 and 25.32 KDa of molecular weight, in the case of puerperal sera those of 82.79, 52.14, 40.91, 37.75, 35.08, 33.52, 28.53, 27.53, and 25.32 KDa, whereas the sera of patients with amebic hepatic abscess those of molecular weight of 719, 118.45, 88.45, 69.45, 55.26, 52.14, 49.38, 42.72, 36.81, 35.08, 33.52, 25.32, 23.82, 23.47, 20.52 and 20.03 KDa. The putative adhesins were recognized infrequently by Iga of puerperal colostra and sera while the IgA of sera of patients with amebic hepatic abscess recognized those of 118.45 KDa (112 KDa in other studies) in 83.33% of the cases.
利用28名产妇初乳和血清样本以及24名诊断为阿米巴肝脓肿患者的血清样本中存在的IgA抗体，对转移至硝酸纤维素纸上的溶组织内阿米巴滋养体的总抗原进行免疫检测。初乳抗体最常识别的抗原分子量分别为1290、82.79、52.14、40.91、37.75、35.08、33.52、28.53、27.53和25.32 kDa；产妇血清识别的抗原分子量为82.79、52.14、40.91、37.75、35.08、33.52、28.53、27.53和25.32 kDa；而阿米巴肝脓肿患者血清识别的抗原分子量为719、118.45、88.45、69.45、55.26、52.14、49.38、42.72、36.81、35.08、33.52、25.32、23.82、23.47、20.52和20.03 kDa。初乳和产妇血清中的IgA很少识别假定的粘附素，而阿米巴肝脓肿患者血清中的IgA在83.33%的病例中识别出分子量为118.45 kDa（其他研究中为112 kDa）的粘附素。
