Tellez Sierra A, Cortez Ruiz L, Aust Kettis A, Huldt G, Jonsson J, Schröder H
University Hospital Oscar Danilo Rosales, León, Nicaragua.
Arch Med Res. 1992;23(2):261-4.
With the aid of the indirect hemagglutination (IHA) test and IgG ELISA the antibody profile against E. histolytica in León, Nicaragua was investigated in 562 sera from individuals belonging to various age groups. The highest reactivity was invariably recorded in the age group 6-15 years where 48% were seropositive. Several sera reactive by either one of IHA and IgG ELISA were negative by the other test. The main reason for this seems to be reactivity in different Ig classes. Treatment with 2-mercaptoethanol reduced the titre level in 63 of the 66 sera tested. Immunofluorescence using an anti-IgM conjugate showed that 26 of 43 sera contained specific IgM-antibodies, indicating that also unspecific reactions are involved in the IHA test. A comparison was made between class-specific reactivity in three population groups: healthy residents, healthy cyst carriers and patients with recent or acute liver abscess. No significant difference in the prevalence of reactions above the diagnostic significance level was recorded between cyst carriers and healthy residents. However, among the cyst carriers 33% had IgA and/or IgM antibodies but no demonstrable specific IgG. Most patients with recent and all with acute liver abscess reacted significantly above the diagnostic limit in all three tests.
借助间接血凝试验（IHA）和IgG酶联免疫吸附测定（ELISA），对尼加拉瓜莱昂不同年龄组562份血清样本中针对溶组织内阿米巴的抗体谱进行了研究。6 - 15岁年龄组的反应性始终最高，其中48%呈血清阳性。IHA和IgG ELISA其中一项呈反应性的几份血清，另一项检测结果为阴性。主要原因似乎是不同Ig类别的反应性。用2 - 巯基乙醇处理后，66份检测血清中的63份滴度水平降低。使用抗IgM缀合物的免疫荧光显示，43份血清中的26份含有特异性IgM抗体，这表明IHA试验中也存在非特异性反应。对三组人群的类别特异性反应性进行了比较：健康居民、健康包囊携带者以及近期或急性肝脓肿患者。包囊携带者和健康居民之间，高于诊断意义水平的反应患病率没有显著差异。然而，在包囊携带者中，33%有IgA和/或IgM抗体，但没有可检测到的特异性IgG。大多数近期肝脓肿患者以及所有急性肝脓肿患者在所有三项检测中的反应均显著高于诊断限值。
