检测海水中、生物基质中以及可兴奋膜上短裸甲藻毒素的方法。

Methods for detecting brevetoxins in seawater, in biological matrices, and on excitable membranes.

作者信息

Baden D G, Schet V M, Rein K S, Edwards R A

机构信息

NIHS Marine and Freshwater Biomedical Sciences Center, University of Miami, Florida 33149.

出版信息

Bull Soc Pathol Exot. 1992;85(5 Pt 2):516-8.

PMID:1340358

Abstract

Although the toxins differ in each of the principal polyether marine seafood intoxication syndromes, developing technology is of general applicability. The brevetoxins can be readily detected in seawater, in dinoflagellate cells, and in shellfish using standardized mouse bioassay, isocratic high performance liquid chromatography, radioimmunoassay and enzyme-linked immunoassay...each possessing distinct advantages and disadvantages. Clinical diagnosis, pharmacokinetics, and membrane-receptor studies on the contrary require specialized radioligand technology and immunofluorescent and immunodense imaging. The applicability of brevetoxins assays towards predictive, detection, and molecular mechanism of action of ciguatoxin is presented.

摘要

尽管在每种主要的聚醚类海洋海鲜中毒综合征中，毒素各不相同，但正在发展的技术具有普遍适用性。使用标准化小鼠生物测定法、等度高效液相色谱法、放射免疫测定法和酶联免疫测定法，可很容易地在海水中、鞭毛藻细胞中和贝类中检测到短裸甲藻毒素……每种方法都有其独特的优缺点。相反，临床诊断、药代动力学和膜受体研究需要专门的放射性配体技术以及免疫荧光和免疫密度成像技术。本文介绍了短裸甲藻毒素检测方法在雪卡毒素预测、检测及作用分子机制方面的适用性。