Dosquet C, Weill D, Wautier J L
UFR Lariboisière, Saint-Louis, Hôpital Lariboisière, Paris, France.
Nouv Rev Fr Hematol (1978). 1992;34 Suppl:S55-9.
The blood monocytes adhere to endothelial cells unstimulated and after stimulation by interleukin-1, tumor necrosis factor or other mediators. This process is mediated through specific molecules on both endothelial cells and monocytes. Using specific monoclonal antibodies and molecular cloning several families of molecules involved in leukocyte endothelial cell interaction have been defined. Leukocyte adhesion molecules include the three beta 2 integrins (CD11/CD18 molecules), VLA-4 and the L-Selectin. E-Selectin (ELAM-1), P-Selectin (GMP-140) and receptors of the immunoglobulin superfamily (ICAM-1, ICAM-2 and VCAM-1) are expressed on endothelial cells in basal conditions and after activation. It has been shown that these adhesive molecules are involved in blood monocyte adhesion to endothelial cells. Monocytes from patients with diabetes mellitus had an increased adhesion to endothelial cells in culture. As estimated by flow cytometry CD11b/CD18 expression on diabetic monocytes was increased. Pentoxifylline reduced CD11b/CD18 expression on normal and diabetic monocytes. This effect was associated to a decrease in monocyte adhesion to endothelial cells.
血液单核细胞在未受刺激以及受到白细胞介素 -1、肿瘤坏死因子或其他介质刺激后均会黏附于内皮细胞。这一过程是通过内皮细胞和单核细胞上的特定分子介导的。利用特异性单克隆抗体和分子克隆技术，已确定了几个参与白细胞与内皮细胞相互作用的分子家族。白细胞黏附分子包括三种β2整合素（CD11/CD18分子）、VLA - 4和L - 选择素。E - 选择素（ELAM - 1）、P - 选择素（GMP - 140）以及免疫球蛋白超家族的受体（ICAM - 1、ICAM - 2和VCAM - 1）在基础状态及激活后在内皮细胞上表达。已表明这些黏附分子参与血液单核细胞与内皮细胞的黏附。糖尿病患者的单核细胞在培养中对内皮细胞的黏附增加。通过流式细胞术估计，糖尿病单核细胞上CD11b/CD18的表达增加。己酮可可碱降低正常和糖尿病单核细胞上CD11b/CD18的表达。这一效应与单核细胞对内皮细胞黏附的减少相关。
