基于β-半乳糖苷酶诱导的大肠杆菌噬菌体检测改进方法。

Improved method for coliphage detection based on beta-galactosidase induction.

作者信息

Ijzerman M M, Hagedorn C

机构信息

Department of Crop and Soil Environmental Sciences, Virginia Polytechnic Institute and State University, Blacksburg 24061-0404.

出版信息

J Virol Methods. 1992 Oct;40(1):31-6. doi: 10.1016/0166-0934(92)90004-w.

DOI:10.1016/0166-0934(92)90004-w

PMID:1341886

Abstract

An improved method for coliphage detection based on the induction of beta-galactosidase in Escherichia coli is described. Upon infection by coliphages, the cells are lysed and a stable indolyl product that is dark blue becomes visible within each plaque. The improved method is compared to the proposed coliphage detection procedure described in Standard Methods for the Examination of Water and Wastewater.

摘要

描述了一种基于大肠杆菌中β-半乳糖苷酶诱导的改进的大肠杆菌噬菌体检测方法。在被大肠杆菌噬菌体感染后，细胞会裂解，并且在每个噬菌斑内会出现一种深蓝色的稳定吲哚产物。将该改进方法与《水和废水检验标准方法》中描述的建议的大肠杆菌噬菌体检测程序进行了比较。