Kenard R P, Valentine R S
Appl Microbiol. 1974 Mar;27(3):484-7. doi: 10.1128/am.27.3.484-487.1974.
A rapid and sensitive method is described for the detection of bacteria in water and various other natural substrates by the isolation of specific bacteriophage. By the addition of large numbers of the organism in question to the sample, the presence of virulent bacteriophage can be demonstrated in as little as 6 to 8 h. Fecal coliform, total coliform, and total coliphage counts were determined for over 150 water samples from several geographical areas over a period of 2 years. Computer analysis of the data shows a high degree of correlation between fecal coliforms and the coliphage present in the samples. With a high correlation coefficient between fecal coliform and coliphage counts, predictions of the fecal coliforms may be made by enumeration of the phage.
本文描述了一种快速灵敏的方法，通过分离特定噬菌体来检测水和各种其他天然基质中的细菌。通过向样品中添加大量相关生物体，可在短短6至8小时内证明烈性噬菌体的存在。在两年时间里，对来自几个地理区域的150多个水样进行了粪大肠菌群、总大肠菌群和总噬菌体计数。对数据的计算机分析表明，样品中的粪大肠菌群与噬菌体之间存在高度相关性。由于粪大肠菌群和噬菌体计数之间的相关系数很高，因此可以通过噬菌体计数来预测粪大肠菌群。
